Evidence map›Paper›PMID 30248415›Full record

ArticleToxicology and applied pharmacology2019

Effect of N-(2-aminoethyl) ethanolamine on hypertrophic scarring changes in vitro: Finding novel anti-fibrotic therapies.

Zhenping Chen, Jianhua Gu, Amina El Ayadi, Andres F Oberhauser, Jia Zhou, Linda E Sousse, Celeste C Finnerty, David N Herndon, Paul J Boor

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology and applied pharmacology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Zhenping ChenDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA.
Jianhua GuAFM/SEM Core Facility, The Houston Methodist Hospital Research Institute, Houston, TX 77030, USA.
Amina El AyadiDepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, TX 77550, USA.
Andres F OberhauserDepartment of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555, USA; Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Jia ZhouDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Linda E SousseDepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, TX 77550, USA.
Celeste C FinnertyDepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, TX 77550, USA.
David N HerndonDepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, TX 77550, USA.
Paul J BoorDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, TX 77550, USA. Electronic address: pboor@UTMB.EDU.
Shriners Hospitals for Children - Galveston · USThe University of Texas Medical Branch at Galveston · USHouston Methodist · US

Funding

Project 9: The role of androgen/corticosteroid in fat, muscle and glucose metabolism of burn victimsP50GM060338 · NIGMS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI SZABO, CSABA · 2000 to 2017
$24.2M
MODULATION OF THE POSTBURN HYPERMETABOLIC RESPONSER01GM056687 · NIGMS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI HERNDON, DAVID N. · 1998 to 2017
$9.0M
Effects of Chronic Catecholamine Exposure on Post-burn ScarringR01GM112936 · NIGMS · UNIVERSITY OF TEXAS MED BR GALVESTON · PI FINNERTY, CELESTE CAMPBELL · 2015 to 2019
$1.8M
GSTs: Oxidative Stress in Early AtherosclerosisR01HL065416 · NHLBI · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI BOOR, PAUL J · 2002 to 2006
$1.5M
Fetal Basis of Dissecting Aortic AneurysmR21ES013038 · NIEHS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI BOOR, PAUL J · 2004 to 2006
$449k
Atomic Force Microscopic Studies of Dissecting AneurysmR21ES022821 · NIEHS · UNIVERSITY OF TEXAS MED BR GALVESTON · PI BOOR, PAUL J · 2013 to 2014
$423k
NHLBI NIH HHS R01 HL065416NIEHS NIH HHS R21 ES013038NIEHS NIH HHS R21 ES022821NIGMS NIH HHS P50 GM060338NIGMS NIH HHS R01 GM056687NIGMS NIH HHS R01 GM112936
6 · The paper itself

Abstract

Hypertrophic scars (HS) limit movement, decrease quality of life, and remain a major impediment to rehabilitation from burns. However, no effective pharmacologic therapies for HS exist. Here we tested the in vitro anti-fibrotic effects of the novel chemical N-(2-aminoethyl) ethanolamine (AEEA) at non-toxic concentrations. Scanning electron microscopy showed that AEEA markedly altered the structure of the extracellular matrix (ECM) produced by primary dermal fibroblasts isolated from a HS of a burn patient (HTS). Compression atomic force microscopy revealed that AEEA stiffened the 3D nanostructure of ECM formed by HTS fibroblasts. Western blot analysis in three separate types of primary human dermal fibroblasts (including HTS) showed that AEEA exposure increased the extractability of type I collagen in a dose- and time-dependent fashion, while not increasing collagen synthesis. A comparison of the electrophoretic behavior of the same set of samples under native and denaturing conditions suggested that AEEA alters the 3D structure of type I collagen. The antagonization effect of AEEA to TGF-β1 on ECM formation was also observed. Furthermore, analyses of the anti-fibrotic effects of analogs of AEEA (with modified pharmacophores) suggest the existence of a chemical structure-activity relationship. Thus, AEEA and its analogs may inhibit HS development; further study and optimization of analogs may be a promising strategy for the discovery for effective HS therapies.

Indexed as

Cell LineCicatrix, HypertrophicCollagenEthanolaminesExtracellular MatrixFibroblastsFibrosisHumansStructure-Activity RelationshipTransforming Growth Factor beta1CollagenEthanolaminesTGFB1 protein, humanTransforming Growth Factor beta1Atomic Force Microscopy (AFM)CollagenDrug DiscoveryScanning Electron Microscopy (SEM)Wound Healing

Identifiers

PMID30248415
PMCPMC6502483
OpenAlexW2893927828

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.