ArticleNature biotechnology2018
A CRISPR-Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 422 papers.
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Who cites it
422 citing papers in PubMed.
- Clinical Safety Experience of TAK-003 for Dengue Fever: A New Tetravalent Live Attenuated Vaccine Candidate.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Trial
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- Article
- Split suppression gene drives reveal a design tradeoff between inheritance and reproductive load inbioRxiv : the preprint server for biology · 2026Article
- Efficient Microinjection and CRISPR/Cas9 Mediated Genome Editing in Urechis unicinctus.Marine biotechnology (New York, N.Y.) · 2026Article
- The nanosd integral gene drive enables population modification of the malaria vector Anopheles gambiae.G3 (Bethesda, Md.) · 2026Article
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae.Genetics · 2026Article
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- Engineered promoter system enables high-efficiency transgenic CRISPR editing in Malaria transmitting mosquitoZoological research · 2026Article
- Local implementation of scalable malaria molecular surveillance in a low-resource setting: a North-South collaborative model.Malaria journal · 2026Article
- Identifying Transgenic Approaches to Mitigate Vector Transmission of Malaria by Anopheles gambiae.Cureus · 2026Review
- Engineering resilient gene drives for sustainable malaria control by predicting, testing and overcoming target site resistance in Anopheles gambiae.PLoS biology · 2026Article
- Harnessing CRISPR-Cas technology for insect pest control: current advances and future perspectives.Molecular biology reports · 2026Review
- Self-limiting population suppression gene drive design in the West Nile vector mosquito, Culex quinquefasciatus.Nature communications · 2026Article
- The architecture of computational antiviralism: a multi-scale framework from molecular targeting to viral ecosystem engineering.Molecular diversity · 2026Review
- Decoding cis-regulatory elements in the germline of the human malaria vector Anopheles gambiae.Communications biology · 2026Article
- Variance partitioning reveals contrasting random effect contributions to the density and species composition of malaria-transmitting mosquitoes in western Burkina Faso.Parasites & vectors · 2026Article
- A simulation-based deep learning framework for spatially explicit malaria modeling of CRISPR suppression gene drive mosquitoes.iScience · 2026Article
- Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae.Nature communications · 2026Article
- Omics Technologies in Molecular Biology.International journal of molecular sciences · 2026Article
362 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male, contains an exon (exon 5) whose sequence is highly conserved in all Anopheles mosquitoes so far analyzed. We found that CRISPR-Cas9-targeted disruption of the intron 4-exon 5 boundary aimed at blocking the formation of functional AgdsxF did not affect male development or fertility, whereas females homozygous for the disrupted allele showed an intersex phenotype and complete sterility. A CRISPR-Cas9 gene drive construct targeting this same sequence spread rapidly in caged mosquitoes, reaching 100% prevalence within 7-11 generations while progressively reducing egg production to the point of total population collapse. Owing to functional constraint of the target sequence, no selection of alleles resistant to the gene drive occurred in these laboratory experiments. Cas9-resistant variants arose in each generation at the target site but did not block the spread of the drive.
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