ArticleJournal of visualized experiments : JoVE2018
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- PDK4 is critical for metabolic regulation and progesterone production in bovine granulosa cells†.Biology of reproduction · 2026Article
- A Novel Culture System for Inhibiting In Vitro Differentiation of Ovine Granulosa Cells.Biomolecules · 2025Article
- Comparative analysis of PI3K-AKT and MEK-ERK1/2 signaling-driven molecular changes in granulosa cells.Reproduction (Cambridge, England) · 2025Article
- Saturated fatty acids inhibit unsaturated fatty acid induced glucose uptake involving GLUT10 and aerobic glycolysis in bovine granulosa cells.Scientific reports · 2024Article
- Early milk-feeding regimes in calves exert long-term effects on the development of ovarian granulosa cells.BMC genomics · 2023Article
- Letrozole administration effects on the P450aromatase expression and the reproductive parameters in male sheep (Animal reproduction · 2023Article
- Redox Status, Estrogen and Progesterone Production by Swine Granulosa Cells Are Impaired by Triclosan.Animals : an open access journal from MDPI · 2022Article
- A rapid and robust method for the cryopreservation of human granulosa cells.Histochemistry and cell biology · 2021Article
- Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress under In Vitro Condition.Animals : an open access journal from MDPI · 2021Article
- Matrix metallopeptidases regulate granulosa cell remodeling through the hormone signaling pathway.Journal of advanced veterinary and animal research · 2020Article
- Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance.Endocrine connections · 2019Article
- L-lactate induces specific genome wide alterations of gene expression in cultured bovine granulosa cells.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ovarian granulosa cells (GC) are the major source of estradiol synthesis. Induced by the preovulatory luteinizing hormone (LH) surge, cells of the theca and, in particular, of the granulosa cell layer profoundly change their morphological, physiological, and molecular characteristics and form the progesterone-producing corpus luteum that is responsible for maintaining pregnancy. Cell culture models are essential tools to study the underlying regulatory mechanisms involved in the folliculo-luteal transformation. The presented protocol focuses on the isolation procedure and cryopreservation of bovine GC from small- to medium-sized follicles (< 6 mm). With this technique, a nearly pure population of GC can be obtained. The cryopreservation procedure greatly facilitates time management of the cell culture work independent of a direct primary tissue (ovaries) supply. This protocol describes a serum-free cell culture model that mimics the estradiol-active status of bovine GC. Important conditions that are essential for a successful steroid-active cell culture are discussed throughout the protocol. It is demonstrated that increasing the plating density of the cells induces a specific response as indicated by an altered gene expression profile and hormone production. Furthermore, this model provides a basis for further studies on GC differentiation and other applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.