Evidence map›Paper›PMID 30247122›Full record

ArticleeLife2018

Generation of a versatile BiFC ORFeome library for analyzing protein-protein interactions in live

Johannes Bischof, Marilyne Duffraisse, Edy Furger, Leiore Ajuria, Guillaume Giraud, Solene Vanderperre, Rachel Paul, Mikael Björklund, Damien Ahr, Alexis W Ahmed and 4 more

Open access · goldAbstract read
In one paragraph

Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 54 citations in OpenAlex.

  1. Single-cell transcriptomics of theProceedings of the National Academy of Sciences of the United States of America · 2026
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  4. Homeodomain Involvement in Nuclear HOX Protein Homo- and Heterodimerization.International journal of molecular sciences · 2025
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  18. Review
  19. Prdx1 Interacts with ASK1 upon Exposure to HAntioxidants (Basel, Switzerland) · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 3 countries.

Johannes Bischof *Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
Marilyne Duffraisse *IGFL, CNRS, ENS Lyon, Lyon, France.
Edy Furger *Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
Leiore Ajuria *IGFL, CNRS, ENS Lyon, Lyon, France.
Guillaume GiraudIGFL, CNRS, ENS Lyon, Lyon, France.
Solene VanderperreIGFL, CNRS, ENS Lyon, Lyon, France.
Rachel PaulIGFL, CNRS, ENS Lyon, Lyon, France.
Mikael BjörklundZhejiang University-University of Edinburgh Institute, Zhejiang University, Haining, China.ORCID http://orcid.org/0000-0002-2176-681X
Damien AhrIGFL, CNRS, ENS Lyon, Lyon, France.
Alexis W AhmedIGFL, CNRS, ENS Lyon, Lyon, France.
Lionel SpinelliINSERM, Aix-Marseille Université, Marseille, France.
Christine BrunINSERM, Aix-Marseille Université, Marseille, France.ORCID https://orcid.org/0000-0002-5563-6765
Konrad BaslerInstitute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
Samir MerabetIGFL, CNRS, ENS Lyon, Lyon, France.ORCID http://orcid.org/0000-0001-7629-703X
École Normale Supérieure de Lyon · FRUniversity of Zurich · CHInserm · FRZhejiang University-University of Edinburgh Institute · CN

Funding

Fondation pour la Recherche Médicale 1122556
6 · The paper itself

Abstract

Transcription factors achieve specificity by establishing intricate interaction networks that will change depending on the cell context. Capturing these interactions in live condition is however a challenging issue that requires sensitive and non-invasive methods. We present a set of fly lines, called 'multicolor BiFC library', which covers most of the

Indexed as

Gene LibraryAnimalsAnimals, Genetically ModifiedColorDrosophilaDrosophila ProteinsEmbryo, NonmammalianFluorescenceGene Expression Regulation, DevelopmentalLuminescent ProteinsMicroscopy, FluorescenceProtein BindingProtein Interaction MappingTranscription FactorsDrosophila ProteinsLuminescent ProteinsTranscription FactorsD. melanogasterfluorescencegeneticsgenomicsHoxprotein interactiontranscription factors

Identifiers

PMID30247122
PMCPMC6177257
OpenAlexW2806726335

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.