ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2018
From Light Microscopy to Analytical Scanning Electron Microscopy (SEM) and Focused Ion Beam (FIB)/SEM in Biology: Fixed Coordinates, Flat Embedding, Absolute References.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- An Improved SEM Preparation Workflow for Plasma Membrane Imaging in HepG2 and IM-9 Cells.Bioengineering (Basel, Switzerland) · 2026Article
- Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria.Biofilm · 2025Article
- Lasso Model-Based Optimization of CNC/CNF/rGO Nanocomposites.Micromachines · 2025Article
- Bulk synthesis of conductive non-metallic carbon nanospheres and a 3D printed carrier device for scanning electron microscope calibration.RSC advances · 2023Article
- CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.The Journal of cell biology · 2023Article
- Evidence of a role for cAMP in mitochondrial regulation in ovarian granulosa cells.Molecular human reproduction · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning.Planta · 2022Article
- Silver Nanoparticles as Potential Antiviral Agents.Pharmaceutics · 2021Review
- Volume electron microscopy: analyzing the lung.Histochemistry and cell biology · 2021Review
- Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution.iScience · 2020Article
- Insights into replicative senescence of human testicular peritubular cells.Scientific reports · 2019Article
- Segmentation and Modelling of the Nuclear Envelope of HeLa Cells Imaged with Serial Block Face Scanning Electron Microscopy.Journal of imaging · 2019Article
- Automated Drawing Tube (Camera Lucida) Method in Light Microscopy Images Analysis Can Comes True.Journal of microscopy and ultrastructureArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Correlative light and electron microscopy (CLEM) has been in use for several years, however it has remained a costly method with difficult sample preparation. Here, we report a series of technical improvements developed for precise and cost-effective correlative light and scanning electron microscopy (SEM) and focused ion beam (FIB)/SEM microscopy of single cells, as well as large tissue sections. Customized coordinate systems for both slides and coverslips were established for thin and ultra-thin embedding of a wide range of biological specimens. Immobilization of biological samples was examined with a variety of adhesives. For histological sections, a filter system for flat embedding was developed. We validated ultra-thin embedding on laser marked slides for efficient, high-resolution CLEM. Target cells can be re-located within minutes in SEM without protracted searching and correlative investigations were reduced to a minimum of preparation steps, while still reaching highest resolution. The FIB/SEM milling procedure is facilitated and significantly accelerated as: (i) milling a ramp becomes needless, (ii) significant re-deposition of milled material does not occur; and (iii) charging effects are markedly reduced. By optimizing all technical parameters FIB/SEM stacks with 2 nm iso-voxels were achieved over thousands of sections, in a wide range of biological samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.