ArticleInternational journal of analytical chemistry2018
Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon.
Article in International journal of analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Near-Infrared Light-Activated DNA Biosensor for Sensitive Detection of miRNAs via a Catalytic Hairpin Assembly Signal Amplification Strategy.ACS omega · 2025Article
- Molecular Beacon for Detection miRNA-21 as a Biomarker of Lung Cancer.International journal of molecular sciences · 2022Article
- A Novel Helper qPCR Assay for the Detection of miRNA Using Target/Helper Template for Primer Formation.International journal of analytical chemistry · 2022Article
- Biological Implications and Clinical Potential of Metastasis-Related miRNA in Colorectal Cancer.Molecular therapy. Nucleic acids · 2021Review
- Corrigendum #2 to "Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon".International journal of analytical chemistry · 2021Article
- Corrigendum to "Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon".International journal of analytical chemistry · 2020Article
- Catalytic hairpin assembly-assisted lateral flow assay for visual determination of microRNA-21 using gold nanoparticles.Mikrochimica acta · 2019Article
- Emerging microRNA biomarkers for colorectal cancer diagnosis and prognosis.Open biology · 2019Review
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA.
Identifiers
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Registered trials
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