ArticleFrontiers in microbiology2018
A Comparative Analysis of Microbial DNA Preparation Methods for Use With Massive and Branching Coral Growth Forms.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Effects of life history strategy on the diversity and composition of the coral holobiont communities of Sabah, Malaysia.Scientific reports · 2025Article
- Selective shaping of prokaryotic communities and core symbiont maintenance suggest large-scale aquarium facilities as reservoirs of microbiome diversity in octocorals.Frontiers in microbiology · 2025Article
- Greater than pH 8: The pH dependence of EDTA as a preservative of high molecular weight DNA in biological samples.PloS one · 2023Article
- Characteristics of The Bleached Microbiome of The Generalist CoralIntegrative organismal biology (Oxford, England) · 2023Article
- Article
- Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics.Frontiers in microbiology · 2022Review
- The maintenance of microbial community in human fecal samples by a cost effective preservation buffer.Scientific reports · 2021Article
- A review of coral bleaching specimen collection, preservation, and laboratory processing methods.PeerJ · 2021Article
- Diverse coral reef invertebrates exhibit patterns of phylosymbiosis.The ISME journal · 2020Article
- DESS deconstructed: Is EDTA solely responsible for protection of high molecular weight DNA in this common tissue preservative?PloS one · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last two decades, over 100 studies have investigated the structure of the coral microbiome. However, as yet there are no standardized methods applied to sample preservation and preparation, with different studies using distinct methods. There have also been several comparisons made of microbiome data generated across different studies, which have not addressed the influence of the methodology employed over each of the microbiome datasets. Here, we assess three different preservation methods; salt saturated dimethyl sulfoxide (DMSO) - EDTA, snap freezing with liquid nitrogen and 4% paraformaldehyde solution, and two different preparation methodologies; bead beating and crushing, that have been applied to study the coral microbiome. We compare the resultant bacterial assemblage data for two coral growth forms, the massive coral
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