ArticleFrontiers in microbiology2018
Investigation of Detection Limits and the Influence of DNA Extraction and Primer Choice on the Observed Microbial Communities in Drinking Water Samples Using 16S rRNA Gene Amplicon Sequencing.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed.
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- Long vs. short read sequencing for microbial ecology of sedimentary environments: a case study from Lake Arnon, Switzerland.FEMS microbiology letters · 2026Article
- Impact of genetically modified herbicide-resistant maize on rhizosphere bacterial communities.GM crops & food · 2025Article
- Metagenomic report of element-microbe synergy and xenobiotic detoxification in the sacred waters of Khecheopalri lake, Eastern Himalaya.BMC microbiology · 2025Article
- Exploring Factors Driving the Uneven Distribution ofMicroorganisms · 2025Article
- The impact of DNA extraction on the quantification ofMicrobiology spectrum · 2024Article
- Enrichment and characterization of human-associated mucin-degrading microbial consortia by sequential passage.FEMS microbiology ecology · 2024Article
- Fine-Tuning of DADA2 Parameters for Multiregional Metabarcoding Analysis of 16S rRNA Genes from Activated Sludge and Comparison of Taxonomy Classification Power and Taxonomy Databases.International journal of molecular sciences · 2024Article
- Improving the efficiency of DNA extraction from iron incrustations and oilfield-produced water.Scientific reports · 2024Article
- Modeling the limits of detection for antimicrobial resistance genes in agri-food samples: a comparative analysis of bioinformatics tools.BMC microbiology · 2024Article
- Enhancing the DNA yield intended for microbial sequencing from a low-biomass chlorinated drinking water.Frontiers in microbiology · 2024Article
- Identifying Eukaryotes and Factors Influencing Their Biogeography in Drinking Water Metagenomes.Environmental science & technology · 2023Article
- Bacterial contamination of medical face mask wearing duration and the optimal wearing time.Frontiers in cellular and infection microbiology · 2023Article
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Environment-Related Genes Analysis ofFoods (Basel, Switzerland) · 2022Article
- The Effects of DNA Extraction Kits and Primers on Prokaryotic and Eukaryotic Microbial Community in Freshwater Sediments.Microorganisms · 2022Article
- Detecting Flavobacterial Fish Pathogens in the Environment via High-Throughput Community Analysis.Applied and environmental microbiology · 2022Article
- Gut Microbiota Diversity of Preterm Neonates Is Associated WithFrontiers in cellular and infection microbiology · 2022Article
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2 authors.
Funding
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Abstract
In recent years, 16S rRNA gene amplicon sequencing has been widely adopted for analyzing the microbial communities in drinking water (DW). However, no comprehensive attempts have been made to illuminate the inherent method biases specifically relating to DW communities. In this study, we investigated the impact of DNA extraction and primer choice on the observed microbial community, and furthermore estimated the detection limit of the 16S rRNA gene amplicon sequencing in these experimental settings. Of the two DNA extraction kits investigated, the PowerWater DNA Isolation Kit resulted in higher yield, better reproducibility and more OTUs identified compared to the FastDNA SPIN Kit for Soil, which is also commonly used within DW microbiome research. The use of three separate primer-sets targeting the V1-3, V3-4, and V4 region of the 16S rRNA gene revealed large differences in OTU abundances, with some of the primers unable to detect entire phyla. Estimations of the detection limit were based on bacteria-free water samples (1 L) spiked with
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