ArticleCell stem cell2018
Esophageal Organoids from Human Pluripotent Stem Cells Delineate Sox2 Functions during Esophageal Specification.
Article in Cell stem cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers, 1 of them a synthesis that pooled it.
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Who cites it
91 citing papers in PubMed, 1 synthesis or guideline pooled it, 154 citations in OpenAlex.
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Large-scale and innervated functional human gut tissues for transplantation via transient spheroid confinement.Nature biomedical engineering · 2026Article
- Closing the gap: Toward functional, bioengineered esophageal grafts.Cell stem cell · 2026Article
- Spatiotemporal Transcriptomic Dissection Uncovers Age-Dependent Deceleration of Esophageal Cell Differentiation.bioRxiv : the preprint server for biology · 2026Article
- Review
- Single-cell transcriptomic comparison of the developing human fetal stomach and pluripotent stem cell-derived gastric organoids.Development (Cambridge, England) · 2026Article
- Metaplastic Barrett's oesophagus represents reversion to a developmental-like epithelial cell state.Development (Cambridge, England) · 2025Article
- Genetic Engineering of Esophageal Organoids: CRISPR-Based Knock-In for Cell Lineage Tracing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A spatiotemporal and machine-learning platform facilitates the manufacturing of hPSC-derived esophageal mucosa.Developmental cell · 2025Article
- Epithelial architecture and signaling activity in the adult human esophagus.Frontiers in cell and developmental biology · 2025Review
- Amniotic fluid modifies esophageal epithelium differentiation and inflammatory responses.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Manipulating cell fate through reprogramming: approaches and applications.Development (Cambridge, England) · 2024Review
- Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids.Cell proliferation · 2024Article
- Research progress and application of liver organoids for disease modeling and regenerative therapy.Journal of molecular medicine (Berlin, Germany) · 2024Review
- Red2Flpe-SCON: a versatile, multicolor strategy for generating mosaic conditional knockout mice.Nature communications · 2024Article
- Jag1/2 maintain esophageal homeostasis and suppress foregut tumorigenesis by restricting the basal progenitor cell pool.Nature communications · 2024Article
- Reciprocal Interactions Between the Epithelium and Mesenchyme in Organogenesis.Results and problems in cell differentiation · 2024Article
- Establishing mouse and human oral esophageal organoids to investigate the tumor immune response.Disease models & mechanisms · 2024Article
- Revolutionising oral organoids with artificial intelligence.Biomaterials translational · 2024Review
- Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon.Cell stem cell · 2023Article
31 more citing papers are in PubMed but not listed here.
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14 authors at 2 institutions in 1 country.
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Abstract
Tracheal and esophageal disorders are prevalent in humans and difficult to accurately model in mice. We therefore established a three-dimensional organoid model of esophageal development through directed differentiation of human pluripotent stem cells. Sequential manipulation of bone morphogenic protein (BMP), Wnt, and RA signaling pathways was required to pattern definitive endoderm into foregut, anterior foregut (AFG), and dorsal AFG spheroids. Dorsal AFG spheroids grown in a 3D matrix formed human esophageal organoids (HEOs), and HEO cells could be transitioned into two-dimensional cultures and grown as esophageal organotypic rafts. In both configurations, esophageal tissues had proliferative basal progenitors and a differentiated stratified squamous epithelium. Using HEO cultures to model human esophageal birth defects, we identified that Sox2 promotes esophageal specification in part through repressing Wnt signaling in dorsal AFG and promoting survival. Consistently, Sox2 ablation in mice causes esophageal agenesis. Thus, HEOs present a powerful platform for modeling human pathologies and tissue engineering.
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