Evidence map›Paper›PMID 30244402›Full record

ArticleLasers in medical science2019

The role of Wnt/β-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation.

In Hwan Cho, Seong Jun Park, Si Hyung Lee, Seung Kwan Nah, Ha Yan Park, Jin Young Yang, Sanjar Batirovich Madrakhimov, Jungmook Lyu, Tae Kwann Park

Abstract read
PubMed Publisher
In one paragraph

Article in Lasers in medical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Rekindling Vision: Innovative Strategies for Treating Retinal Degeneration.International journal of molecular sciences · 2025
    Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

In Hwan ChoDepartment of Ophthalmology, Soonchunhyang University Hospital Cheonan, Cheonan, Republic of Korea.
Seong Jun ParkCollege of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
Si Hyung LeeDepartment of Ophthalmology, Soonchunhyang University Hospital Bucheon, 170 Jomaru-ro, Wonmi-gu, Bucheon, 14584, Republic of Korea.
Seung Kwan NahDepartment of Ophthalmology, Soonchunhyang University Hospital Bucheon, 170 Jomaru-ro, Wonmi-gu, Bucheon, 14584, Republic of Korea.
Ha Yan ParkDepartment of Ophthalmology, Soonchunhyang University Hospital Bucheon, 170 Jomaru-ro, Wonmi-gu, Bucheon, 14584, Republic of Korea.
Jin Young YangDepartment of Biomedical science, Graduate School, Soonchunhyang University, Bucheon, Republic of Korea.
Sanjar Batirovich MadrakhimovDepartment of Biomedical science, Graduate School, Soonchunhyang University, Bucheon, Republic of Korea.
Jungmook LyuDepartment of Medical Science, Konyang University, 685 Gasoowon-dong, Seo-gu, Daejeon, 302-718, Republic of Korea. lyujm5@gmail.com.
Tae Kwann ParkCollege of Medicine, Soonchunhyang University, Cheonan, Republic of Korea. tkpark@schmc.ac.kr.ORCID http://orcid.org/0000-0001-9689-4384
Soonchunhyang University · KRSoonchunhyang University Hospital Seoul · KRKonyang University · KR

Funding

National Research Foundation of Korea 2016R1A2B4008376
6 · The paper itself

Abstract

To investigate the role of Wnt/β-catenin signaling pathway in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE) after laser photocoagulation. After differentiation of RPE cells from hiPSCs, laser photocoagulation was performed. Activation of Wnt/β-catenin signaling at days 1 and 5 after laser photocoagulation was evaluated by expression of β-catenin. Cell proliferation and alteration in cell-to-cell contact at day 5 after laser photocoagulation with or without Dickkopf-1 (Dkk-1) treatment were studied using ethynyl-2'-deoxyuridine (EdU) assay and zonula occludens-1 (ZO-1) expression analysis, respectively. The mRNA levels of Wnt genes at day 5 after laser photocoagulation were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Activation of Wnt/β-catenin signaling at days 1 and 5 after laser photocoagulation was confirmed by β-catenin accumulation in the cytoplasm and nucleus of hiPSC-RPE. Many EdU-positive cells also expressed β-catenin, and the number of EdU-positive cells was decreased at day 5 after laser photocoagulation after Dkk-1 treatment, indicating that Wnt/β-catenin signaling mediated hiPSC-RPE proliferation. ZO-1 expression was not decreased with Dkk-1 treatment at day 5 after laser photocoagulation, indicating that Wnt/β-catenin signaling mediated hiPSC-RPE restoration. At day 5, after laser photocoagulation, mRNA levels of Wnt2b, Wnt3, Wnt5a, Wnt7a, and Wnt10b were increased. Wnt/β-catenin signaling has a crucial role in restoration of hiPSC-RPE proliferation after laser photocoagulation. Manipulation of Wnt/β-catenin signaling while elucidating the underlying mechanisms of RPE restoration might have a therapeutic potential in retinal degenerative diseases.

Indexed as

Laser CoagulationWnt Signaling Pathwaybeta CateninCell DifferentiationCell ProliferationCell ShapeFluorescenceGene Expression RegulationHumansInduced Pluripotent Stem CellsIntercellular Signaling Peptides and ProteinsRetinal Pigment EpitheliumRNA, MessengerTime FactorsZonula Occludens-1 Proteinbeta CateninDKK1 protein, humanIntercellular Signaling Peptides and ProteinsRNA, MessengerZonula Occludens-1 ProteinInduced pluripotent stem cellLaser photocoagulationRetinal pigment epitheliumWnt/β-catenin signaling pathway

Identifiers

PMID30244402
OpenAlexW2894295005

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.