ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2018
Fine mapping and candidate gene screening of the downy mildew resistance gene RPF1 in Spinach.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
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- Dual transcriptional characterization of spinach and Peronospora effusa during resistant and susceptible race-cultivar interactions.BMC genomics · 2024Article
- Identification of Clubroot (Genes · 2024Article
- Skim resequencing finely maps the downy mildew resistance lociHorticulture research · 2023Article
- Fine Mapping and Identification of a Candidate Gene of Downy Mildew Resistance,International journal of molecular sciences · 2022Article
- Genetic mapping, transcriptomic sequencing and metabolic profiling indicated a glutathione S-transferase is responsible for the red-spot-petals in Gossypium arboreum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Resequencing worldwide spinach germplasm for identification of field resistance QTLs to downy mildew and assessment of genomic selection methods.Horticulture research · 2022Article
- Mapping and selection of downy mildew resistance in spinach cv. whale by low coverage whole genome sequencing.Frontiers in plant science · 2022Article
- Genomic analyses provide insights into spinach domestication and the genetic basis of agronomic traits.Nature communications · 2021Article
- High resolution mapping and candidate gene identification of downy mildew race 16 resistance in spinach.BMC genomics · 2021Article
- A spinach genome assembly with remarkable completeness, and its use for rapid identification of candidate genes for agronomic traits.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Genome-wide simple sequence repeats (SSR) markers discovered from whole-genome sequence comparisons of multiple spinach accessions.Scientific reports · 2021Article
- Fine mapping and molecular marker development of the Fs gene controlling fruit spines in spinach (Spinacia oleracea L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Integration of early disease-resistance phenotyping, histological characterization, and transcriptome sequencing reveals insights into downy mildew resistance in impatiens.Horticulture research · 2021Article
- Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi.BMC plant biology · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inBioMed research international · 2021Article
- Phenotypic Diversity and Association Mapping of Ascorbic Acid Content in Spinach.Frontiers in genetics · 2021Article
- A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.).Scientific reports · 2020Article
- Transcriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew.Scientific reports · 2020Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
key messageA SLAF-BSA approach was used to locate the RPF1 locus. The three most likely candidate genes were identified which provide a basic for cloning the resistance gene at the RPF1 locus. Spinach downy mildew is a globally devastating oomycete disease. The use of downy mildew resistance genes constitutes the most effective approach for disease management. Hence, the objective of the present study was to fine map the first-reported resistance locus RPF1. The resistance allele at this resistance locus was effective against races 1-7, 9, 11, 13, and 15 of Peronospora farinosa f. sp. spinaciae (P. effusa). The approach fine mapped RPF1 using specific-locus amplified fragment sequencing (SLAF-Seq) technology combined with bulked segregant analysis. A 1.72 Mb region localized on chromosome 3 was found to contain RPF1 based on association analysis. After screening recombinants with the SLAF markers within the region, the region was narrowed down to 0.89 Mb. Within this region, 14 R genes were identified based on the annotation information. To identify the genes involved in resistance, resequencing of two resistant inbred lines (12S2 and 12S3) and three susceptible inbred lines (12S1, 12S4, and 10S2) was performed. The three most likely candidate genes were identified via amino acid sequence analysis and conserved domain analysis between resistant and susceptible inbred lines. These included Spo12729, encoding a receptor-like protein, and Spo12784 and Spo12903, encoding a nucleotide-binding site and leucine-rich repeat domains. Additionally, based on the sequence variation in the three genes between the resistant and susceptible lines, molecular markers were developed for marker-assisted selection. The results could be valuable in cloning the RPF1 alleles and improving our understanding of the interaction between the host and pathogen.
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