Evidence map›Paper›PMID 30242599›Full record

ArticleEndocrine2019

MIR4532 gene variant rs60432575 influences the expression of KCNJ11 and the sulfonylureas-stimulated insulin secretion.

Zhang-Ren Chen, Fa-Zhong He, Mou-Ze Liu, Jin-Lei Hu, Heng Xu, Hong-Hao Zhou, Wei Zhang

Abstract read
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In one paragraph

Article in Endocrine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Zhang-Ren ChenDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China.
Fa-Zhong HeDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China.
Mou-Ze LiuDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China.
Jin-Lei HuDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China.
Heng XuState Key Laboratory of Biotherapy, Sichuan University, Chengdu, 610041, Sichuan, China.
Hong-Hao ZhouDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China.
Wei ZhangDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China. yjsd2003@163.com.
PharmacoGenetics (China) · CNCentral South University · CNSichuan University · CNXiangya Hospital Central South University · CN

Funding

Central South University Innovation Foundation for Postgraduate 2016zzts518Innovation Driven Project of Central South University 2016CX024National High Technology Research and Development Program of China 2012AA02A518National Key Research and Development Program 2016YFC0905000National Natural Science Foundation of China 81522048, 81573511, 81273595
6 · The paper itself

Abstract

purposeDiabetes mellitus is a major chronic disease and causes over one million deaths. KCNJ11 genetic polymorphisms influence the response of first-line oral antidiabetic agent sulfonylureas. Hsa-miR-4532 correlates with diabetic nephropathy and has a high abundance in urine. MIR4532 rs60452575 G>A variant changes the mature sequence of hsa-miR-4532. We studied whether the genetic polymorphisms of MIR4532 rs60452575 would influence KCNJ11 expression and sulfonylurea-stimulated insulin secretion or not.

methodsTo estimate the influence that rs60452575 G>A variant has on the interaction of hsa-miR-4532 and KCNJ11, we constructed a pmirGLO vector containing 3' UTR of KCNJ11 and co-transfected it with wild-type and mutant hsa-miR-4532 mimics into HEK293 cells; and we overexpressed wild-type and mutant hsa-miR-4532 mimics into HEK293 cells and MIN6 cells to access its effects on KCNJ11 expression and response of sulfonylureas.

resultsMIR4532 rs60452575 G>A variant appeared to disrupt the repression of KCNJ11 expression in both cell lines, and reduce the sulfonylurea-stimulated insulin secretion by breaking the binding of the hsa-miR-4532 to 3' UTR of KCNJ11 in MIN6 cells.

conclusionsOur study indicates that MIR4532 rs60452575 variant influences KCNJ11 expression and sulfonylurea response. It might be a potential predictive factor of sulfonylureas therapy.

Indexed as

Insulin Secretion3' Untranslated RegionsHEK293 CellsHumansKcnj11 ChannelMicroRNAsPolymorphism, Single NucleotidePotassium Channels, Inwardly RectifyingSulfonylurea Compounds3' Untranslated RegionsKcnj11 ChannelMicroRNAsMIRN4532 microRNA, humanPotassium Channels, Inwardly RectifyingSulfonylurea CompoundsDiabetesInsulinKCNJ11MicroRNAPolymorphismSulfonylureas

Identifiers

PMID30242599
OpenAlexW2891345973

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.