ArticlePloS one2018
Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 30 citations in OpenAlex.
- Insights into intraspecific variation and genotyping ofIMA fungus · 2026Article
- Unraveling bioactive potential and production inChinese herbal medicines · 2025Review
- Review on mushroom mycelium-based products and their production process: from upstream to downstream.Bioresources and bioprocessing · 2025Review
- Application of Miscanthus substrates in the cultivation of Ganoderma lingzhi.Archives of microbiology · 2023Article
- The Effect of Mitochondria onJournal of fungi (Basel, Switzerland) · 2022Article
- Nonthermal Plasma Effects on Fungi: Applications, Fungal Responses, and Future Perspectives.International journal of molecular sciences · 2022Review
- Application of Non-Thermal Plasma to Fungal Resources.Journal of fungi (Basel, Switzerland) · 2022Review
- Interspecific hybridization between Ganoderma lingzhi and G. applanatum through protoplast fusion.World journal of microbiology & biotechnology · 2021Article
- A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of L-cysteine.Analytical and bioanalytical chemistry · 2021Article
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9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.
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