ArticleNucleic acids research2018
Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- How RAG1/2 evolved from ancestral transposases to initiate V(D)J recombination without transposition.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Characterization of hAT DNA transposon superfamily in the genome of Neotropical fish Apareiodon sp.Molecular genetics and genomics : MGG · 2024Article
- Multiple horizontal transfer events of a DNA transposon into turtles, fishes, and a frog.Mobile DNA · 2024Article
- A Unifying Hypothesis for the Genome Dynamics Proposed to Underlie Neuropsychiatric Phenotypes.Genes · 2024Review
- Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding.Nature communications · 2023Article
- Massive Somatic and Germline Chromosomal Integrations of Polydnaviruses in Lepidopterans.Molecular biology and evolution · 2023Article
- Inner workings of RAG recombinase and its specialization for adaptive immunity.Current opinion in structural biology · 2021Review
- Article
- Structures of ISCth4 transpososomes reveal the role of asymmetry in copy-out/paste-in DNA transposition.The EMBO journal · 2021Article
- How mouse RAG recombinase avoids DNA transposition.Nature structural & molecular biology · 2020Article
- Cutting antiparallel DNA strands in a single active site.Nature structural & molecular biology · 2020Article
- Jump ahead with a twist: DNA acrobatics drive transposition forward.Current opinion in structural biology · 2019Review
- Article
- Structure of the P element transpososome reveals new twists on the DD(E/D) theme.Nature structural & molecular biology · 2019Article
- Snapshots of a genetic cut-and-paste.Nature · 2019Article
- Transposons to V(D)J Recombination: Evolution of the RAG Reaction.Trends in immunology · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Some DNA transposons relocate from one genomic location to another using a mechanism that involves generating double-strand breaks at their transposon ends by forming hairpins on flanking DNA. The same double-strand break mode is employed by the V(D)J recombinase at signal-end/coding-end junctions during the generation of antibody diversity. How flanking hairpins are formed during DNA transposition has remained elusive. Here, we describe several co-crystal structures of the Hermes transposase bound to DNA that mimics the reaction step immediately prior to hairpin formation. Our results reveal a large DNA conformational change between the initial cleavage step and subsequent hairpin formation that changes which strand is acted upon by a single active site. We observed that two factors affect the conformational change: the complement of divalent metal ions bound by the catalytically essential DDE residues, and the identity of the -2 flanking base pair. Our data also provides a mechanistic link between the efficiency of hairpin formation (an A:T basepair is favored at the -2 position) and Hermes' strong target site preference. Furthermore, we have established that the histidine residue within a conserved C/DxxH motif present in many transposase families interacts directly with the scissile phosphate, suggesting a crucial role in catalysis.
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