Evidence map›Paper›PMID 30239276›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2018

Development and evaluation of a multiplex reverse-transcription real-time PCR assay for detection of equine respiratory disease viruses.

Shimaa M Ghoniem, Ayman H El Deeb, Mohammed G Aggour, Hussein A Hussein

Open access · bronzeAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Insect-specific Alphamesonivirus-1 (Journal of virology · 2025
    Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Shimaa M GhoniemDepartments of Virology (Ghoniem), Animal Health Research Institute, Dokki, Egypt.
Ayman H El DeebDepartments of Virology (Ghoniem), Animal Health Research Institute, Dokki, Egypt.
Mohammed G AggourDepartments of Virology (Ghoniem), Animal Health Research Institute, Dokki, Egypt.
Hussein A HusseinDepartments of Virology (Ghoniem), Animal Health Research Institute, Dokki, Egypt.
Cairo University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a multiplex reverse-transcription real-time PCR (RT-rtPCR) assay for the simultaneous detection of the main equine respiratory viruses: equid alphaherpesviruses 1 and 4 (EHV-1, -4) and equine influenza virus (EIV; species Influenza A virus). The primers and probes amplified only the targeted viruses, and there were no inter-assay cross-amplifications or nonspecific interactions. The multiplex assay efficiencies were 92.5%, 97%, and 90% for EHV-1, EHV-4, and EIV, respectively. The R

Indexed as

AnimalsDNA PrimersHerpesviridae InfectionsHerpesvirus 1, EquidHerpesvirus 4, EquidHorse DiseasesHorsesInfluenza A virusMultiplex Polymerase Chain ReactionOrthomyxoviridae InfectionsReal-Time Polymerase Chain ReactionRespiratory Tract DiseasesSensitivity and SpecificityDNA PrimersEquinemultiplex real-time PCRrespiratory viruses

Identifiers

PMID30239276
PMCPMC6505849
OpenAlexW2891598287

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.