ArticleBirth defects research2019
Tissue biomechanics during cranial neural tube closure measured by Brillouin microscopy and optical coherence tomography.
Article in Birth defects research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 58 citations in OpenAlex.
- Off-Axis Illumination and Epicollection Confocal Brillouin Scattering Microspectroscopy.Analytical chemistry · 2026Article
- Discovering the unexpected: Insights into the dynamics of mouse neural tube closure revealed by time-lapse imaging.Developmental biology · 2026Review
- Imaging the mechanical properties of a developing embryonic tendon using Brillouin microscopy.Acta biomaterialia · 2025Article
- A lateral tension model for mouse cranial neural tube closure.bioRxiv : the preprint server for biology · 2025Article
- Design of neural organoids engineered by mechanical forces.IBRO neuroscience reports · 2024Review
- Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.Development (Cambridge, England) · 2024Article
- In vivo dynamic volumetric imaging of mouse testis and epididymis with optical coherence tomography†.Biology of reproduction · 2024Article
- Brillouin microscopy.Nature reviews. Methods primers · 2024Article
- Monitoring the Mechanical Evolution of Tissue During Neural Tube Closure of Chick Embryo.Journal of visualized experiments : JoVE · 2023Article
- Porcine vocal fold elasticity evaluation using Brillouin spectroscopy.Journal of biomedical optics · 2023Article
- Versatile multimodal modality based on Brillouin light scattering and the photoacoustic effect.Optics letters · 2023Article
- Toward peripheral nerve mechanical characterization using Brillouin imaging spectroscopy.Neurophotonics · 2023Article
- A versatile multimodal optical modality based on Brillouin light scattering and photoacoustic effect.bioRxiv : the preprint server for biology · 2023Article
- Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy.Scientific reports · 2023Article
- Review
- Following the Beat: Imaging the Valveless Pumping Function in the Early Embryonic Heart.Journal of cardiovascular development and disease · 2022Review
- Multimodal imaging system combining optical coherence tomography and Brillouin microscopy for neural tube imaging.Optics letters · 2022Article
- Mouse embryo phenotyping with optical coherence tomography.Frontiers in cell and developmental biology · 2022Review
- The emerging role of mechanical and topographical factors in the development and treatment of nervous system disorders: dark and light sides of the force.Pharmacological reports : PR · 2021Review
- Neuromechanobiology: An Expanding Field Driven by the Force of Greater Focus.Advanced healthcare materials · 2021Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundEmbryonic development involves the interplay of driving forces that shape the tissue and the mechanical resistance that the tissue offers in response. While increasing evidence has suggested the crucial role of physical mechanisms underlying embryo development, tissue biomechanics is not well understood because of the lack of techniques that can quantify the stiffness of tissue in situ with 3D high-resolution and in a noncontact manner.
methodsWe used two all-optical techniques, optical coherence tomography (OCT) and Brillouin microscopy, to map the longitudinal modulus of the tissue from mouse embryos in situ.
resultsWe acquired 2D mechanical maps of the neural tube region of embryos at embryonic day (E) 8.5 (n = 2) and E9.5 (n = 2) with submicron spatial resolution. We found the modulus of tissue varied distinctly within the neural tube region of the same embryo and between embryos at different development stages, suggesting our technique has enough sensitivity and spatial resolution to monitor the tissue mechanics during embryonic development in a noncontact and noninvasive manner.
conclusionsWe demonstrated the capability of OCT-guided Brillouin microscopy to quantify tissue longitudinal modulus of mouse embryos in situ, and observed distinct change in the modulus during the closure of cranial neural tube. Although this preliminary work cannot provide definitive conclusions on biomechanics of neural tube closure yet as a result of the limited number of samples, it provides an approach of quantifying the tissue mechanics during embryo development in situ, thus could be helpful in investigating the role of tissue biomechanics in the regulation of embryonic development. Our next study involving more embryo samples will investigate systematic changes in tissue mechanics during embryonic development.
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