Evidence map›Paper›PMID 30239166›Full record

ArticleMicrobiologyOpen2019

Strong in combination: Polyphasic approach enhances arguments for cold-assigned cyanobacterial endemism.

Patrick Jung, Laura Briegel-Williams, Michael Schermer, Burkhard Büdel

Open access · goldAbstract read
In one paragraph

Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
7.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Patrick JungPlant Ecology and Systematics, Biology Institute, University of Kaiserslautern, Kaiserslautern, Germany.ORCID 0000-0002-7607-3906
Laura Briegel-WilliamsPlant Ecology and Systematics, Biology Institute, University of Kaiserslautern, Kaiserslautern, Germany.ORCID 0000-0003-3926-8840
Michael SchermerPlant Ecology and Systematics, Biology Institute, University of Kaiserslautern, Kaiserslautern, Germany.
Burkhard BüdelPlant Ecology and Systematics, Biology Institute, University of Kaiserslautern, Kaiserslautern, Germany.
University of Kaiserslautern · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyanobacteria of biological soil crusts (BSCs) represent an important part of circumpolar and Alpine ecosystems, serve as indicators for ecological condition and climate change, and function as ecosystem engineers by soil stabilization or carbon and nitrogen input. The characterization of cyanobacteria from both polar regions remains extremely important to understand geographic distribution patterns and community compositions. This study is the first of its kind revealing the efficiency of combining denaturing gradient gel electrophoresis (DGGE), light microscopy and culture-based 16S rRNA gene sequencing, applied to polar and Alpine cyanobacteria dominated BSCs. This study aimed to show the living proportion of cyanobacteria as an extension to previously published meta-transcriptome data of the same study sites. Molecular fingerprints showed a distinct clustering of cyanobacterial communities with a close relationship between Arctic and Alpine populations, which differed from those found in Antarctica. Species richness and diversity supported these results, which were also confirmed by microscopic investigations of living cyanobacteria from the BSCs. Isolate-based sequencing corroborated these trends as cold biome clades were assigned, which included a potentially new Arctic clade of Oculatella. Thus, our results contribute to the debate regarding biogeography of cyanobacteria of cold biomes.

Indexed as

BiodiversitySoil MicrobiologyAntarctic RegionsArctic RegionsBacteriological TechniquesCluster AnalysisCold TemperatureCyanobacteriaDenaturing Gradient Gel ElectrophoresisDNA, BacterialDNA, RibosomalMicroscopyPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNATundraDNA, BacterialDNA, RibosomalRNA, Ribosomal, 16SAntarcticaArcticbiogeographybiological soil crustscyanobacteriadenaturing gradient gel electrophoresisendemismpolyphasic approach

Identifiers

PMID30239166
PMCPMC6528576
OpenAlexW2909156670

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.