Evidence map›Paper›PMID 30238933›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2018

MicroRNA let-7c-5p Suppressed Lipopolysaccharide-Induced Dental Pulp Inflammation by Inhibiting Dentin Matrix Protein-1-Mediated Nuclear Factor kappa B (NF-κB) Pathway In Vitro and In Vivo.

Hao Yuan, Hong Zhang, Lihua Hong, Hongyan Zhao, Jiafeng Wang, He Li, Hongze Che, Zhimin Zhang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 43 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Hao YuanDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Hong ZhangDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Lihua HongDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Hongyan ZhaoDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Jiafeng WangDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
He LiDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Hongze CheDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Zhimin ZhangDepartment of Endodontics, School of Stomatology, Jilin University, Changchun, Jilin, China (mainland).
Jilin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND Let-7c-5p is down-regulated in dental pulp tissues in inflammatory disorders. The microRNA (miR) molecule shows an anti-inflammation potential due to its direct regulation of dentin matrix protein-1 (DMP1), which promotes inflammation changes in dental pulp tissues. In the present study, the effect of let-7c-5p on lipopolysaccharide (LPS)-induced pulpitis was detected and the associated mechanism was explored. MATERIAL AND METHODS Dental pulp stem cells (DPSCs) were isolated from rat dental tissues, infected with let-7c-5p lentivirus particles, and subjected to LPS administration to induce inflammation. Then, the effect of let-7c-5p overexpression on LPS-induced impairments on DPSCs were detected and the mechanism was explained by focusing on the DMP1 expression and NF-κB pathway. The role of DMP1 in the anti-inflammation effect of let-7c-5p was assessed by incubating let-7c-5p-expressed DPSCs with DMP1 protein. The results of in vitro assays were verified in LPS-induced rat pulpitis models. RESULTS LPS administration increased the production of IL-1β and TNF-α and decreased DPSCs viability by increasing the expression of DMP1 and activating NF-κB pathway. However, the induced expression of let-7c-5p relieved DPSCs from LPS-induced inflammation and suppressed DMP1 as well as NF-κB pathway. The incubation of let-7c-5p-expressed DPSCs with DMP1 protein blocked the effect of let-7c-5p. In in vivo experiments, the injection of let-7c-5p attenuated LPS-induced pulpitis by inhibiting DMP1-mediated NF-κB pathway. CONCLUSIONS Findings outlined in the current study demonstrated the dental pulp protecting function of let-7c-5p during LPS-induced inflammation, which was exerted by inhibiting the DMP1-mediated NF-κB pathway.

Indexed as

AnimalsCell DifferentiationCells, CulturedCell SurvivalDental PulpDentinExtracellular Matrix ProteinsInflammationInterleukin-1betaLipopolysaccharidesMicroRNAsNF-kappa BPhosphoproteinsRatsRats, Sprague-DawleySignal TransductionDmp1 protein, ratExtracellular Matrix ProteinsInterleukin-1betaLipopolysaccharidesMicroRNAsMIRNLET7 microRNA, ratNF-kappa BPhosphoproteins

Identifiers

PMID30238933
PMCPMC6162970
OpenAlexW2890214308

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.