ArticleAMB Express2018
Molecular identification of some wild Nigerian mushrooms using internal transcribed spacer: polymerase chain reaction.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Molecular identification of some wild macrofungi from Southeastern Nigeria.BMC genomic data · 2026Article
- Characterization and Identification of an Antimicrobial Compound Psilocybin from Psychedelic Mushroom.Indian journal of microbiology · 2025Review
- Efficient PCR - based Approach for Rapid Identification of Earth Star Mushrooms Employing Species-Specific Primers.Mycoscience · 2025Article
- Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes.Scientific reports · 2022Article
- Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds.Journal, genetic engineering & biotechnology · 2021Article
- Molecular characterization of most cultivatedHeliyon · 2021Article
- Cultivation and Nutritional Value of ProminentMycobiology · 2020Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)-polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected at Environmental Pollution Science and Technology farm, Ilesa, Southwest Nigeria. The PCR amplification of ITS1 and 4 of the mushrooms isolates yielded approximately 850 bp. Amplicons obtained were sequenced and identified using BLASTn in the NCBI. The BLASTn results revealed that Termitomyces aurantiacus (3), Tricholoma matsutake (8), Tricholoma robustum (2), P. ostreatus (4), Schizophyllum commune (1) and Pleurotus pulmonarius (1) were fully represented. Only Tricholoma matsutake (KT273371), Pleurotus pulmonarius (KY962469) and Tricholoma matsutake (AF438605) had 100% similarity with reference strain. However, the phylogenetic analysis of the isolates showed low genetic relatedness with reference strains. This study revealed the novelty of the mushroom strains and thus advocating the need for strict conservation measures and further investigations on their potential benefits to mankind.
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