ArticlePloS one2018
Comprehensive molecular characterization of microneedling therapy in a human three-dimensional skin model.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Cell-Free Blood Cell Secretome (BCS) Counteracts Skin Aging: Multi-Center Prospective Regenerative Aesthetic Medicine Study Using Exokine®.Clinical, cosmetic and investigational dermatology · 2022Trial
- Novel dermis niche equivalent based on a 3D-structure fabricated by 2-photon polymerization.Biomaterials and biosystems · 2026Article
- Transcriptomic responses of skin to different micro-injury modalities: fire filiform needling, ablative fractional COLasers in medical science · 2026Article
- Development and Optimization of Retinyl Palmitate-Loaded Oleogel and Nanoemulsion Designed with Potential for Microneedling Treatment.Pharmaceuticals (Basel, Switzerland) · 2026Article
- A reproducible mechano-thermal injury method for reconstructed human epidermis to study wound re-epithelialization.MethodsX · 2026Article
- Mechanically Induced Skin Renewal: Evidence-Based Insights Into Clinical, Structural and Inflammatory Changes Activated by Microneedling, Microdermabrasion and Microvibration.Journal of cosmetic dermatology · 2026Review
- Deciphering molecular responses to radiofrequency microneedling in a 3D human skin model: new insights and modulation by aftercare treatment.Frontiers in medicine · 2026Article
- The Application of a Formulation Containing Hydroxypropyl Tetrahydropyrantriol and Niacinamide to Augment Anti-aging Benefits in a Double-blind, Randomized Nonablative Laser Treatment Study.The Journal of clinical and aesthetic dermatology · 2025Article
- Platelet-Released Growth Factors (PRGFs) Activate NRF2-ARE and Modulate Inflammatory Response in an NRF2-Dependent Manner in Primary Human Keratinocytes.Journal of cosmetic dermatology · 2025Article
- A standardized in vitro bioengineered skin for penetrating wound modeling.In vitro models · 2025Article
- Tattooed human in vitro skin model for testing the biocompatibility of tattoo inks and healing progression after tattooing.Scientific reports · 2025Article
- Candidate SNP Markers Significantly Altering the Affinity of the TATA-Binding Protein for the Promoters of Human Genes Associated with Primary Open-Angle Glaucoma.International journal of molecular sciences · 2024Article
- Management of Melasma: Laser and Other Therapies-Review Study.Journal of clinical medicine · 2024Review
- Continuous Glucose Monitoring Enabled by Fluorescent Nanodiamond Boronic Hydrogel.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Progress of polymer-based strategies in fungal disease management: Designed for different roles.Frontiers in cellular and infection microbiology · 2023Review
- Post-Treatment of Micro-Needling with a Dexpanthenol-Containing Ointment Accelerates Epidermal Wound Healing in Human 3D Skin Models.Clinical, cosmetic and investigational dermatology · 2023Article
- Efficacy and tolerability of a microneedling device for treating wrinkles on the face.Journal of cosmetic dermatology · 2023Article
- Approaches in line with human physiology to prevent skin aging.Frontiers in physiology · 2023Review
- Enhancement of recombinant human IL-24 (rhIL-24) protein production from site-specific integrated engineered CHO cells by sodium butyrate treatment.Bioprocess and biosystems engineering · 2022Article
- Utilization of ex vivo tissue model to study skin regeneration following microneedle stimuli.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesMicroneedling therapy is a widely used technique in dermatology. However, little is known about the underlying molecular effects of this therapy on extracellular matrix remodeling, wound healing, and inflammation. The aim of this study was to examine morphological and molecular changes caused by microneedling treatment in a standardized in vitro full-thickness 3D model of human skin. MATERIALS AND
methodsA microneedling device was used to treat full-thickness 3D skin models. Specimens were harvested at specified time points and qRT-PCR and microarray studies were performed. Frozen sections were examined histologically.
resultsMicroneedling treatment caused morphological changes in the skin model resulting in an almost complete recovery of the epidermis five days after treatment. Microarray analysis identified an upregulation of genes that are associated with tissue remodeling and wound healing (e.g. COL3A1, COL8A1, TIMP3), epithelial proliferation and differentiation (KRT13, IGF1), immune cell recruitment (CCL11), and a member of the heat shock protein family (HSPB6). On the other hand, we detected a downregulation of pro-inflammatory cytokines (e.g. IL1α, IL1β, IL24, IL36γ, IL36RN), and antimicrobial peptides (e.g. S100A7A, DEFB4). These data were confirmed by independent RT-PCR analyses.
conclusionWe present for the first time the direct molecular effects of microneedling therapy on epidermal keratinocytes and dermal fibroblasts using a standardized 3D skin model. Treatment resulted in histological alterations and changed the expression of various genes related to epidermal differentiation, inflammation, and dermal remodeling. This data suggests that skin microneedling plays a role in dermal remodeling, increases epidermal differentiation, and might also have a direct effect on collagen synthesis. These findings may increase our understanding of the molecular mechanisms of human skin repair induced by microneedling therapy and will allow comparisons with competing applications, such as ablative laser therapies.
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