ArticlePloS one2018
Pseudomonas aeruginosa displays a dormancy phenotype during long-term survival in water.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Enumeration Method forMicroorganisms · 2026Article
- Role of starvation survival response mechanisms on ribosome integrity, antibiotic tolerances, and virulence of Pseudomonas aeruginosa biofilms.NPJ biofilms and microbiomes · 2026Article
- Dormancy in Non-Spore-Forming Bacteria: A Review.Microorganisms · 2025Review
- Veterinary Clinics as Reservoirs forAntibiotics (Basel, Switzerland) · 2025Review
- Modern antiseptics against multidrug-resistantFrontiers in microbiology · 2025Article
- An outbreak ofInfection prevention in practice · 2024Article
- Genetics of conditional extended mycelial cell viability ofFrontiers in microbiology · 2024Article
- Unveiling the Catalytic Mechanism of a Processive Metalloaminopeptidase.Biochemistry · 2023Article
- Resistance inAntibiotics (Basel, Switzerland) · 2023Review
- A CcdB toxin-derived peptide acts as a broad-spectrum antibacterial therapeutic in infected mice.EMBO reports · 2023Article
- Impact of Chlorine Dioxide on Pathogenic Waterborne Microorganisms Occurring in Dental Chair Units.Microorganisms · 2023Article
- The First Swedish Outbreak with VIM-2-ProducingMicroorganisms · 2023Article
- The emergency discharge of sewage to the Bay of Gdańsk as a source of bacterial enrichment in coastal air.Scientific reports · 2021Article
- Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom.Transcription · 2021Review
- From genotype to phenotype: adaptations ofMicrobial genomics · 2021Review
- Contribution of Drugs Interfering with Protein and Cell Wall Synthesis to the Persistence ofInternational journal of molecular sciences · 2021Article
- Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump.Antimicrobial agents and chemotherapy · 2021Article
- Triclosan depletes the membrane potential in Pseudomonas aeruginosa biofilms inhibiting aminoglycoside induced adaptive resistance.PLoS pathogens · 2020Article
- Review
- Rapid determination of carbapenem resistance by low-cost colorimetric methods: Propidium Iodide and alamar blue staining.Journal of microbiology (Seoul, Korea) · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is capable of long-term survival in water, which may serve as a reservoir for infection. Although viable cell counts of PAO1 incubated in water remain stable throughout 8 weeks, LIVE/DEAD staining indicated a high proportion of cells stained with propidium iodide (PI). The proportion of PI-stained cells increased by 4 weeks, then decreased again by 8 weeks, suggesting an adaptive response. This was also evident in an observed shift in cell morphology from a rod to a coccoid shape after 8 weeks. Fluorescence-activated cell sorting (FACS) was used to recover PI-stained cells, which were plated and shown to be viable, indicating that PI-stained cells were membrane-compromised but still cultivable. PAO1 mid-log cells in water were labeled with the dsDNA-binding dye PicoGreen to monitor viability as well as DNA integrity, which demonstrated that the population remains viable and transitions towards increased dsDNA staining. Metabolic activity was found to decrease significantly in water by 4 weeks. The PAO1 outer membrane became less permeable and more resistant to polymyxin B damage in water, and the profile of total membrane lipids changed over time. Among the ~1400 transcriptional lux fusions, gene expression in water revealed that the majority of genes were repressed, but subsets of genes were induced at particular time points. In summary, these results indicate that P. aeruginosa is dormant in water and this adaptation involves a complex pattern of gene regulation and changes to the cell to promote long-term survival and antibiotic tolerance. The approach of P. aeruginosa incubated in water may be useful to study antibiotic tolerance and the mechanisms of dormancy and survival in nutrient limiting conditions.
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