ArticleFrontiers in molecular biosciences2018
Quantification of Intrinsically Disordered Proteins: A Problem Not Fully Appreciated.
Article in Frontiers in molecular biosciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Walking the tightrope: Balancing opposing cooperativities in dynein assembly.The Journal of biological chemistry · 2026Article
- Walking the Tightrope: Balancing Opposing Cooperativities as an Operating Principle in Dynein Assembly.bioRxiv : the preprint server for biology · 2026Article
- Coacervate droplet sequestration of heterogenous nanoplastics with elastin-like polypeptides.bioRxiv : the preprint server for biology · 2026Article
- Self-association and multimer formation in AtLEA4-5, a desiccation-induced intrinsically disordered protein from plants.Protein science : a publication of the Protein Society · 2024Article
- Deciphering Hyperammonia-Producing Bacteria (HAB) in the Rumen of Water Buffaloes (Microorganisms · 2024Article
- Structural study of the intrinsically disordered tardigrade damage suppressor protein (Dsup) and its complex with DNA.Scientific reports · 2024Article
- Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.ACS measurement science au · 2024Review
- Alternative conformations of a group 4 Late Embryogenesis Abundant protein associated to its in vitro protective activity.Scientific reports · 2024Article
- Fluorescent molecular rotors as versatile in situ sensors for protein quantitation.Scientific reports · 2023Article
- Article
- Quantifying cooperative multisite binding in the hub protein LC8 through Bayesian inference.PLoS computational biology · 2023Article
- Chitosan Increases Lysine Content through Amino Acid Transporters inFoods (Basel, Switzerland) · 2022Article
- Molecular dynamics simulations of the adsorption of an intrinsically disordered protein: Force field and water model evaluation in comparison with experiments.Frontiers in molecular biosciences · 2022Article
- Revealing Intrinsic Disorder and Aggregation Properties of the DPF3a Zinc Finger Protein.ACS omega · 2021Article
- Cloning, expression and purification of the low-complexity region of RanBP9 protein.Protein expression and purification · 2020Article
- NMR Lineshape Analysis of Intrinsically Disordered Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- Conserved Glycines Control Disorder and Function in the Cold-Regulated Protein, COR15A.Biomolecules · 2019Article
- Structural Plasticity of Intrinsically Disordered LEA Proteins fromFrontiers in plant science · 2019Article
- Troubleshooting Guide to Expressing Intrinsically Disordered Proteins for Use in NMR Experiments.Frontiers in molecular biosciences · 2018Review
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12 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Protein quantification is essential in a great variety of biochemical assays, yet the inherent systematic errors associated with the concentration determination of intrinsically disordered proteins (IDPs) using classical methods are hardly appreciated. Routinely used assays for protein quantification, such as the Bradford assay or ultraviolet absorbance at 280 nm, usually seriously misestimate the concentrations of IDPs due to their distinct and variable amino acid composition. Therefore, dependable method(s) have to be worked out/adopted for this task. By comparison to elemental analysis as the gold standard, we show through the example of four globular proteins and nine IDPs that the ninhydrin assay and the commercial Qubit
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