ArticleFrontiers in chemistry2018
Collagen Partition in Polymeric Aqueous Two-Phase Systems for Tissue Engineering.
Article in Frontiers in chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- First implementation of a polymer-polymer aqueous two-phase system to improve lateral-flow immunoassay sensitivity via dextranase integration on paper.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Advancements in Aqueous Two-Phase Systems for Enzyme Extraction, Purification, and Biotransformation.Molecules (Basel, Switzerland) · 2024Review
- Phase-separation facilitated one-step fabrication of multiscale heterogeneous two-aqueous-phase gel.Nature communications · 2023Article
- Leveraging ultra-low interfacial tension and liquid-liquid phase separation in embedded 3D bioprinting.Biophysics reviews · 2022Review
- Recovery Techniques Enabling Circular Chemistry from Wastewater.Molecules (Basel, Switzerland) · 2022Review
- Optimized MALDI-TOF MS Strategy for Characterizing Polymers.Frontiers in chemistry · 2021Article
- Higher-order structure of DNA determines its positioning in cell-size droplets under crowded conditions.PloS one · 2021Article
- Self-Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid-Liquid Phase Separation.Chembiochem : a European journal of chemical biology · 2020Article
- Three-dimensional models of breast cancer-fibroblasts interactions.Experimental biology and medicine (Maywood, N.J.) · 2020Review
- Organotypic breast tumor model elucidates dynamic remodeling of tumor microenvironment.Biomaterials · 2020Article
- Kinetic Analysis of Label-Free Microscale Collagen Gel Contraction Using Machine Learning-Aided Image Analysis.Frontiers in bioengineering and biotechnology · 2020Article
- Fabrication of Human Keratinocyte Cell Clusters for Skin Graft Applications by Templating Water-in-Water Pickering Emulsions.Biomimetics (Basel, Switzerland) · 2019Article
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2 authors.
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Abstract
Aqueous two-phase systems (ATPS) of polyethylene glycol (PEG) and dextran (DEX) are commonly used to partition proteins. Protein partition in ATPS is a complex phenomenon and depends on factors including molecular weight of polymers, and electrochemical and ionic properties of the phases. We studied the effect of polymer molecular weight on the partition of a natural protein, collagen, in several ATPS formulations made with non-ionic polymers polyethylene glycol (PEG) and dextran (DEX). We found that partitioning of collagen to an aqueous phase significantly increases when the molecular weight of the corresponding phase polymer decreases. Additionally, a large difference between the molecular weight of the phase-forming polymers was necessary to cause a significant uneven collagen distribution between the aqueous phases. We then employed one of the systems to create a three-dimensional breast cancer microtissue by entrapping a spheroid of breast cancer cells within the partitioned collagen. This convenient technique to generate 3D microtissues offers a convenient and promising approach for tissue engineering applications.
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