ArticleThe Journal of cell biology1986
Dynamics of membrane-skeleton (fodrin) organization during development of polarity in Madin-Darby canine kidney epithelial cells.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
85 citing papers in PubMed, 247 citations in OpenAlex.
- A Na,K-ATPase-Fodrin-Actin Membrane Cytoskeleton Complex is Required for Endothelial Fenestra Biogenesis.Cells · 2020Article
- Article
- Directional Fluid Transport across Organ-Blood Barriers: Physiology and Cell Biology.Cold Spring Harbor perspectives in biology · 2017Review
- E-cadherin polarity is determined by a multifunction motif mediating lateral membrane retention through ankyrin-G and apical-lateral transcytosis through clathrin.The Journal of biological chemistry · 2013Article
- The spectrin cytoskeleton is crucial for adherent and invasive bacterial pathogenesis.PloS one · 2011Article
- Adducins regulate remodeling of apical junctions in human epithelial cells.Molecular biology of the cell · 2010Article
- Membrane domains based on ankyrin and spectrin associated with cell-cell interactions.Cold Spring Harbor perspectives in biology · 2009Review
- A Golgi-associated protein 4.1B variant is required for assimilation of proteins in the membrane.Journal of cell science · 2009Article
- Coordinated protein sorting, targeting and distribution in polarized cells.Nature reviews. Molecular cell biology · 2008Review
- Adducin promotes micrometer-scale organization of beta2-spectrin in lateral membranes of bronchial epithelial cells.Molecular biology of the cell · 2008Article
- Sodium pump localization in epithelia.Journal of bioenergetics and biomembranes · 2007Review
- The roles of the Na,K-ATPase beta 1 subunit in pump sorting and epithelial integrity.Journal of bioenergetics and biomembranes · 2007Review
- A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity.The Journal of cell biology · 2007Article
- AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Functional links between membrane transport and the spectrin cytoskeleton.The Journal of membrane biology · 2006Review
- TGF-beta, neuronal stem cells and glioblastoma.Oncogene · 2005Review
- EphA kinase activation regulates HGF-induced epithelial branching morphogenesis.The Journal of cell biology · 2003Article
- Mutations in beta-spectrin disrupt axon outgrowth and sarcomere structure.The Journal of cell biology · 2000Article
- Drosophila beta spectrin functions independently of alpha spectrin to polarize the Na,K ATPase in epithelial cells.The Journal of cell biology · 2000Article
- Biogenesis of polarized epithelial cells during kidney development in situ: roles of E-cadherin-mediated cell-cell adhesion and membrane cytoskeleton organization.Molecular biology of the cell · 1998Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Madin-Darby canine kidney (MDCK) epithelial cells exhibit a polarized distribution of membrane proteins between the apical and basolateral domains of the plasma membrane. We have initiated studies to investigate whether the spectrin-based membrane skeleton plays a role in the establishment and maintenance of these membrane domains. MDCK cells express an isoform of spectrin composed of two subunits, Mr 240,000 (alpha-subunit) and Mr 235,000 (gamma-subunit). This isoform is immunologically and structurally related to fodrin in lens and brain cells, which is a functional and structural analog of alpha beta-spectrin, the major component of the erythrocyte membrane skeleton. Analysis of fodrin in MDCK cells by immunoblotting, immunofluorescence, and metabolic labeling revealed significant changes in the biophysical properties, subcellular distribution, steady-state levels, and turnover of the protein during development of a continuous monolayer of cells. The changes in the cellular organization of fodrin did not appear to coincide with the distributions of microfilaments, microtubules, or intermediate filaments. These changes result in the formation of a highly insoluble, relatively dense and stable layer of fodrin which appears to be localized to the cell periphery and predominantly in the region of the basolateral plasma membrane of MDCK cells in continuous monolayers. The formation of this structure coincides temporally and spatially with extensive cell-cell contact, and with the development of the polarized distribution of the Na+, K+-ATPase, a marker protein of the basolateral plasma membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.