ArticleJournal of thoracic disease2018
Oxidative damage and DNA damage in lungs of an ovalbumin-induced asthmatic murine model.
Article in Journal of thoracic disease, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Iron-related Genes and Proteins Involved in Iron Homeostasis in Animal Models of Allergic Asthma: A Systematic Review.Biological trace element research · 2025Pooled it
- Untargeted metabolomics profiling of childhood asthma: linking metabolic pattern to disease severity.Molecular and cellular pediatrics · 2026Article
- Molecular Duality of OGG1: From Genomic Guardian to Redox-Sensitive Modulator in Diseases.Antioxidants (Basel, Switzerland) · 2025Review
- Identification of hub genes related to DNA damage response in asthma via combinative bioinformatics strategy.The Journal of international medical research · 2025Article
- Ischemia-reperfusion injury after spinal cord decompressive surgery-An in vivo rat model.Animal models and experimental medicine · 2025Article
- β-glucan nanoparticles alleviate acute asthma by suppressing ferroptosis and DNA damage in mice.Apoptosis : an international journal on programmed cell death · 2025Article
- Article
- Implications of DNA damage in chronic lung disease.Frontiers in cell and developmental biology · 2024Review
- Soybean glycinin and β-conglycinin damage the intestinal barrier by triggering oxidative stress and inflammatory response in weaned piglets.European journal of nutrition · 2023Article
- Severe COVID-19 classified by simple covid risk index is associated with higher levels of advanced oxidation protein products and 8-hydroxy 2 deoxyguanosine.Epidemiology and infection · 2023Article
- Effect of 8-Hydroxyguanine DNA Glycosylase 1 on the Function of Immune Cells.Antioxidants (Basel, Switzerland) · 2023Review
- The gut microbiota of people with asthma influences lung inflammation in gnotobiotic mice.iScience · 2023Article
- DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction.Journal of advanced research · 2022Article
- Integrated analysis of mRNA and long noncoding RNA profiles in peripheral blood mononuclear cells of patients with bronchial asthma.BMC pulmonary medicine · 2022Article
- Associations between Levels of Urinary Oxidative Stress of 8-OHdG and Risk of Atopic Diseases in Children.International journal of environmental research and public health · 2020Article
- Oxidative damage mediates the association between polycyclic aromatic hydrocarbon exposure and lung function.Environmental health : a global access science source · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAsthma is characterized to chronic airway inflammation. However, the role of oxidative damage and DNA damage in the pathophysiology of asthma have rarely been studied. On the other hand, there are evidences that DNA-dependent protein kinase (DNA-PK) participates in DNA damage repair and regulates innate immune responses and proinflammatory signaling pathways.
methodsAfter ovalbumin (OVA)-induced asthmatic murine model was established, airway hyper-responsiveness (AHR), total and differential bronchoalveolar lavage fluid (BALF) cell counts. IL-4, IL-8, IL-13 and TNF-α were chosen to evaluate the airway inflammation, and oxidative damage indicators levels (8-isoprostane and 8-OhdG) in BALF were measured. Alkaline comet assay was conducted to detected DNA damage. Histological analysis was conducted after hematoxylin and eosin (HE) straining, and proteins were extracted for 3-nitrotyrosine (3-NT) detection and immunoblotting.
resultsAHR, infiltration of inflammatory cells and pro-inflammatory cytokine levels in lungs were significantly higher in asthmatic mice. OVA challenge resulted in robust increase in 3-NT, 8-isoprostane and 8OHdG in lungs, which represented oxidative damage level. DNA damage and repair proteins levels in asthma were also increased. NU7441 aggravated the DNA damage level. However, it suppressed infiltration of lung inflammatory cells and inflammatory cytokine levels, suggesting that DNA-PK may be a potential target for treatment of allergic asthma.
conclusionsOur study showed that oxidative damage and DNA damage existed in the airway of asthmatic mice. NU7441 augmented DNA damage level, and moreover, it also attenuated infiltration of inflammatory cells and pro-inflammatory cytokine levels in asthmatic lungs.
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Registered trials
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