ArticlePloS one2018
Discovery of microRNA-like RNAs during early fruiting body development in the model mushroom Coprinopsis cinerea.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Fungal Hydrophobins: Taxonomic Distribution, Functional Roles, Physicochemical Properties, and Biotechnological Applications.Journal of fungi (Basel, Switzerland) · 2026Review
- New insights into temperature-impacted mycovirus-fungus interactions regulated by a microRNA inJournal of virology · 2025Article
- Global discovery, expression pattern, and regulatory role of miRNA-like RNAs inFrontiers in microbiology · 2025Article
- Global Analysis of microRNA-like RNAs Reveals Differential Regulation of Pathogenicity and Development inJournal of fungi (Basel, Switzerland) · 2024Article
- Advancements in genetic studies of mushrooms: a comprehensive review.World journal of microbiology & biotechnology · 2024Review
- The Genome-Wide Characterization of Alternative Splicing and RNA Editing in the Development ofJournal of fungi (Basel, Switzerland) · 2023Article
- Lessons on fruiting body morphogenesis from genomes and transcriptomes ofStudies in mycology · 2023Article
- OMICS and Other Advanced Technologies in Mycological Applications.Journal of fungi (Basel, Switzerland) · 2023Review
- Advances of exosomal miRNAs in the diagnosis and treatment of ovarian cancer.Discover oncology · 2023Review
- MicroRNA-like RNA Functions Are Required for the Biosynthesis of Active Compounds in the Medicinal FungusMicrobiology spectrum · 2022Article
- Evolutionary Morphogenesis of Sexual Fruiting Bodies in Basidiomycota: Toward a New Evo-Devo Synthesis.Microbiology and molecular biology reviews : MMBR · 2022Review
- Article
- Discovery of microRNA-like Small RNAs in Pathogenic Plant FungusInternational journal of molecular sciences · 2022Article
- Identification and physiological function of one microRNA (Mycoscience · 2021Article
- The Evolutionary Significance of RNAi in the Fungal Kingdom.International journal of molecular sciences · 2020Review
- Unmatched Level of Molecular Convergence among Deeply Divergent Complex Multicellular Fungi.Molecular biology and evolution · 2020Article
- Chilling Stress Triggers VvAgo1-Mediated miRNA-Like RNA Biogenesis inFrontiers in microbiology · 2020Article
- Degradation of Fungal MicroRNAs Triggered by Short Tandem Target Mimics Is via the Small-RNA-Degrading Nuclease.Applied and environmental microbiology · 2019Article
- milR4 and milR16 Mediated Fruiting Body Development in the Medicinal FungusFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coprinopsis cinerea is a model mushroom particularly suited for the study of fungal fruiting body development and the evolution of multicellularity in fungi. While microRNAs (miRNAs) have been extensively studied in animals and plants for their essential roles in post-transcriptional regulation of gene expression, miRNAs in fungi are less well characterized and their potential roles in controlling mushroom development remain unknown. To identify miRNA-like RNAs (milRNAs) in C. cinerea and explore their expression patterns during the early developmental transition of mushroom development, small RNA libraries of vegetative mycelium and primordium were generated and putative milRNA candidates were identified following the standards of miRNA prediction in animals and plants. Two out of 22 novel predicted milRNAs, cci-milR-12c and cci-milR-13e-5p, were validated by northern blot and stem-loop reverse transcription real-time PCR. Cci-milR-12c was differentially expressed whereas the expression levels of cci-milR-13e-5p were similar in the two developmental stages. Target prediction of the validated milRNAs resulted in genes associated with fruiting body development, including pheromone, hydrophobin, cytochrome P450, and protein kinase. Essential genes for miRNA biogenesis, including three coding for Dicer-like (DCL), one for Argonaute (AGO), one for AGO-like and one for quelling deficient-2 (QDE-2) proteins, were also identified in the C. cinerea genome. Phylogenetic analysis showed that the DCL and AGO proteins of C. cinerea were more closely related to those in other basidiomycetes and ascomycetes than to those in animals and plants. Taken together, our findings provided the first evidence for milRNAs in the model mushroom and their potential roles in regulating fruiting body development. New information on the evolutionary relationship of milRNA biogenesis proteins across kingdoms has also provided new insights for guiding further functional and evolutionary studies of miRNAs.
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