ArticleMolecular microbiology2018
Inorganic polyphosphate interacts with nucleolar and glycosomal proteins in trypanosomatids.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- Novel binding partners of the Vacuolar Transporter Chaperone (VTC) complex in Acidocalcisomes of Leishmania tarentolae.PLoS neglected tropical diseases · 2026Article
- Article
- Probing metazoan polyphosphate biology usingeLife · 2026Article
- Article
- Article
- Reentrant DNA shells tune polyphosphate condensate size.Nature communications · 2024Article
- Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology inbioRxiv : the preprint server for biology · 2024Article
- Inorganic Polyphosphate Is in the Surface ofPathogens (Basel, Switzerland) · 2024Article
- A mammalian model reveals inorganic polyphosphate channeling into the nucleolus and induction of a hyper-condensate state.Cell reports methods · 2024Article
- Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes.Microbiology and molecular biology reviews : MMBR · 2024Review
- Reentrant DNA shells tune polyphosphate condensate size.bioRxiv : the preprint server for biology · 2023Article
- How much (ATP) does it cost to build a trypanosome? A theoretical study on the quantity of ATP needed to maintain and duplicate a bloodstream-form Trypanosoma brucei cell.PLoS pathogens · 2023Article
- New insights into the role of acidocalcisomes in trypanosomatids.The Journal of eukaryotic microbiology · 2022Review
- Bacterial-Type Long-Chain Polyphosphates Bind Human Proteins in the Phosphatidylinositol Signaling Pathway.Thrombosis and haemostasis · 2022Article
- Ddp1 Cooperates with Ppx1 to Counter a Stress Response Initiated by Nonvacuolar Polyphosphate.mBio · 2022Article
- Delineating transitions during the evolution of specialised peroxisomes: Glycosome formation in kinetoplastid and diplonemid protists.Frontiers in cell and developmental biology · 2022Article
- Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- Hysteresis of pyruvate phosphate dikinase from Trypanosoma cruzi.Parasitology research · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Inorganic polyphosphate (polyP) is a polymer of three to hundreds of phosphate units bound by high-energy phosphoanhydride bonds and present from bacteria to humans. Most polyP in trypanosomatids is concentrated in acidocalcisomes, acidic calcium stores that possess a number of pumps, exchangers, and channels, and are important for their survival. In this work, using polyP as bait we identified > 25 putative protein targets in cell lysates of both Trypanosoma cruzi and Trypanosoma brucei. Gene ontology analysis of the binding partners found a significant over-representation of nucleolar and glycosomal proteins. Using the polyphosphate-binding domain (PPBD) of Escherichia coli exopolyphosphatase (PPX), we localized long-chain polyP to the nucleoli and glycosomes of trypanosomes. A competitive assay based on the pre-incubation of PPBD with exogenous polyP and subsequent immunofluorescence assay of procyclic forms (PCF) of T. brucei showed polyP concentration-dependent and chain length-dependent decrease in the fluorescence signal. Subcellular fractionation experiments confirmed the presence of polyP in glycosomes of T. brucei PCF. Targeting of yeast PPX to the glycosomes of PCF resulted in polyP hydrolysis, alteration in their glycolytic flux and increase in their susceptibility to oxidative stress.
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