ArticleMikrochimica acta2018
A PCR microreactor machinery with passive micropump and battery-powered heater for thermo-cycled amplifications of clinical-level and multiplexed DNA targets.
Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed.
- Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases.Medicine in novel technology and devices · 2022Review
- Cloud-Based Software Architecture for Fully Automated Point-of-Care Molecular Diagnostic Device.Sensors (Basel, Switzerland) · 2021Article
- Cost-Effective Multiplex Fluorescence Detection System for PCR Chip.Sensors (Basel, Switzerland) · 2021Article
- Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene.Micromachines · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A self-contained polymerase chain reaction (PCR) platform with miniaturized power-system is introduced. It is powered by portable lithium batteries and integrated continuous-flow PCR amplification platform. Generally speaking, traditional commercial thermal cyclers rely on external electric supply and thus they are too big in instrument size. This prevents real-timely and field testing during PCR diagnosis. The authors are introducing a continuous-flow 3D spiral microreactor for DNA amplifications and high-resolution multiplexed targets' detection by utilizing the polyvinyl chloride (PVC) tubing-polymer to fabricate the microreactor for the first time. The whole setup (that can all be placed in one hand) includes (a) the thermo-cycled control (5.5 cm width, 10 cm length and 11 cm height), (b) the passive continuous-flow control, and (c) the trapezoidal PCR microreactor. The PCR platform can work for 4.5 h continuously. With minimal accessories and operations, the total cost of the self-contained PCR machinery is <20 $, much lower than the mainstream of commercial PCR machinery. By waiving external electric supply, this miniaturized PCR platform is applied to amplify the typical DNA fragments of plasma isolated hepatitis B virus (HBV), influenza virus (H7N9avian influenza) bacterium (Escherichia coli) plasmid and multiplexed targets. The efficiency of the method is 70% of that of commercial thermal cycler (CFX Connect, Bio Rad). The DNA of H7N9avian influenza can be detected in concentrations as low as 10
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