ArticleData in brief2018
Protein carbonylation detection methods: A comparison.
Article in Data in brief, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
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Who cites it
16 citing papers in PubMed.
- Prenatal Bisphenol A Exposure Impairs Fetal Heart Development: Molecular and Structural Alterations with Sex-Specific Differences.Antioxidants (Basel, Switzerland) · 2025Article
- Explainable Deep Multilevel Attention Learning for Predicting Protein Carbonylation Sites.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Scars of oxidative stress: protein carbonylation and beta cell dysfunction in diabetes.Frontiers in endocrinology · 2025Review
- Early Handling Exerts Anxiolytic Effects and Alters Brain Mitochondrial Dynamics in Adult High Anxiety Mice.Molecular neurobiology · 2024Article
- Protein Carbonylation as a Biomarker of Oxidative Stress and a Therapeutic Target in Neonatal Brain Damage.Antioxidants (Basel, Switzerland) · 2023Review
- Targeting protein modifications in metabolic diseases: molecular mechanisms and targeted therapies.Signal transduction and targeted therapy · 2023Review
- The Role of Potential Oxidative Biomarkers in the Prognosis of Acute Ischemic Stroke and the Exploration of Antioxidants as Possible Preventive and Treatment Options.International journal of molecular sciences · 2023Review
- Protein carbonylation in food and nutrition: a concise update.Amino acids · 2022Article
- Positive Aspects of Oxidative Stress at Different Levels of the Human Body: A Review.Antioxidants (Basel, Switzerland) · 2022Review
- Approaches for Reactive Oxygen Species and Oxidative Stress Quantification in Epilepsy.Antioxidants (Basel, Switzerland) · 2020Review
- Detection, identification, and quantification of oxidative protein modifications.The Journal of biological chemistry · 2019Review
- Malondialdehyde interferes with the formation and detection of primary carbonyls in oxidized proteins.Redox biology · 2019Article
- Revisiting Oxidative Stress and the Use of Organic Selenium in Dairy Cow Nutrition.Animals : an open access journal from MDPI · 2019Review
- Mitochondrial membrane anchored photosensitive nano-device for lipid hydroperoxides burst and inducing ferroptosis to surmount therapy-resistant cancer.Theranostics · 2019Article
- Current Probes for Imaging Carbonylation in Cellular Systems and Their Relevance to Progression of Diseases.Technology in cancer research & treatmentReview
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The data reported here are a comparison among four different methods for the detection of carbonylated proteins, a validated biomarker of oxidative stress. The reference samples were heart and kidney extracts of Guinea pigs transfused with hemoglobin-based oxygen carriers (Alomari et al. FRBM, [11]). We measured the carbonyl content of organ extracts by using i) the Levine spectrophotometric method, which takes advantage of the chromogenic reaction of carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH), ii) a commercially available ELISA assay based on an anti-DNPH antibodies, iii) a commercially available Western blot method based on anti-DNPH antibodies and iv) an in-gel detection approach with the fluorophoric reagent fluorescein-5-thiosemicarbazide. The former two methods measure total protein carbonylation of a sample, whereas the latter two require an electrophoretic separation and therefore potentially allow for the identification of specific carbonylated proteins.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.