Evidence map›Paper›PMID 30228193›Full record

ArticleMolecular & cellular proteomics : MCP2018

Chromobodies to Quantify Changes of Endogenous Protein Concentration in Living Cells.

Bettina-Maria Keller, Julia Maier, Kathy-Ann Secker, Stefanie-Maria Egetemaier, Yana Parfyonova, Ulrich Rothbauer, Bjoern Traenkle

Abstract read
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
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  5. Article
  6. Peptide-Tag Specific Nanobodies for Studying Proteins in Live Cells.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
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  14. Review
  15. Applying Antibodies Inside Cells: Principles and Recent Advances in Neurobiology, Virology and Oncology.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2020
    Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bettina-Maria KellerPharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany.
Julia MaierPharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany.
Kathy-Ann SeckerPharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany.
Stefanie-Maria EgetemaierPharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany.
Yana ParfyonovaPharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany.
Ulrich RothbauerPharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany; Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany. Electronic address: ulrich.rothbauer@uni-tuebingen.de.
Bjoern TraenklePharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany. Electronic address: bjoern.traenkle@nmi.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding cellular processes requires the determination of dynamic changes in the concentration of genetically nonmodified, endogenous proteins, which, to date, is commonly accomplished by end-point assays

Indexed as

AntibodiesAntigen-Antibody ComplexAntigensbeta CateninFluorescent Antibody TechniqueHeLa CellsHumansLysosomesPoint MutationProteasome Endopeptidase ComplexProtein BindingProtein StabilityProteolysisSingle-Domain AntibodiesUbiquitinAntibodiesAntigen-Antibody ComplexAntigensbeta CateninCTNNB1 protein, humanProteasome Endopeptidase ComplexSingle-Domain AntibodiesUbiquitinAntibodiesCell biologychromobodyFluorescenceImagingImaging Visualization ToolsMolecular ProbesnanobodyN-terminal modificationsProtein DegradationProtein TurnoverUbiquitin

Identifiers

PMID30228193
PMCPMC6283300

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.