Evidence map›Paper›PMID 30227960›Full record

ArticleiScience2018

High-Throughput Fitness Profiling of Zika Virus E Protein Reveals Different Roles for Glycosylation during Infection of Mammalian and Mosquito Cells.

Danyang Gong, Tian-Hao Zhang, Dawei Zhao, Yushen Du, Travis J Chapa, Yuan Shi, Laurie Wang, Deisy Contreras, Gang Zeng, Pei-Yong Shi and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
5.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 39 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Evolution of Zika virus inVirus evolution · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Article
  16. Article
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  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Danyang GongDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Tian-Hao ZhangDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Dawei ZhaoDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Yushen DuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Travis J ChapaDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Yuan ShiDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Laurie WangDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
Deisy ContrerasBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Gang ZengDepartment of Urology, University of California, Los Angeles, CA 90095, USA.
Pei-Yong ShiDepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Ting-Ting WuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Vaithilingaraja ArumugaswamiBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Ren SunDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA. Electronic address: rsun@mednet.ucla.edu.
University of California, Los Angeles · USCedars-Sinai Medical Center · USThe University of Texas Medical Branch at Galveston · USYale University · US

Funding

Virology CoreP01CA177322 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WU, TING-TING · 2014 to 2018
$8.7M
Develop a therapeutic vaccine approach by removing viral immune evasionR01DE023591 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SUN, REN · 2013 to 2017
$1.9M
NCI NIH HHS P01 CA177322NIDCR NIH HHS R01 DE023591
6 · The paper itself

Abstract

Zika virus (ZIKV) infection causes Guillain-Barré syndrome and severe birth defects. ZIKV envelope (E) protein is the major viral protein involved in cell receptor binding and entry and is therefore considered one of the major determinants in ZIKV pathogenesis. Here we report a gene-wide mapping of functional residues of ZIKV E protein using a mutant library, with changes covering every nucleotide position. By comparing the replication fitness of every viral mutant between mosquito and human cells, we identified that mutations affecting glycosylation display the most divergence. By characterizing individual mutants, we show that ablation of glycosylation selectively benefits ZIKV infection of mosquito cells by enhancing cell entry, whereas it either has little impact on ZIKV infection on certain human cells or leads to decreased infection through the entry factor DC-SIGN. In conclusion, we define the roles of individual residues of ZIKV envelope protein, which contribute to ZIKV replication fitness in human and mosquito cells.

Indexed as

EntomologyGenomicsProtein Structure AspectsVirology

Identifiers

PMID30227960
PMCPMC6135943
OpenAlexW2764218871

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.