ArticleMetabolism: clinical and experimental2018
Limitations of platform assays to measure serum 25OHD level impact on guidelines and practice decision making.
Article in Metabolism: clinical and experimental, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Upward changes in serum 25-hydroxyvitamin D levels in South Korea: the Korea National Health and Nutrition Examination Survey studies IX (2022-2023) versus VI (2013-2014).Osteoporosis and sarcopenia · 2026Article
- A Serum and Urine Metabolomics Dataset of TCM Syndromes in Cholelithiasis.Scientific data · 2026Article
- Plasma metabolomics dataset of race-walking athletes illuminating systemic metabolic reaction of exercise.Scientific data · 2025Article
- The association of combined vitamin C and D deficiency with bone mineral density and vertebral fracture.Journal of orthopaedic surgery and research · 2024Article
- Optimisation of vitamin D status in global populations.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2024Review
- Vitamin D Measurement: Clinical Practice and Research Implications.The journal for nurse practitioners : JNP · 2023Article
- Article
- 25-Hydroxyvitamin D assay standardisation and vitamin D guidelines paralysis.Public health nutrition · 2020Article
- Vitamin D testing: advantages and limits of the current assays.European journal of clinical nutrition · 2020Review
- Serum 25-Hydroxyvitamin D Concentrations in Patients with Type 2 Diabetes Mellitus in Eastern Sudan: A Case-Control Study.SAGE open nursingArticle
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
contextLiquid Chromatography Mass Spectroscopy (LC-MS/MS) is the preferred method to measure 25 hydroxyvitamin D (25OHD) levels, but laboratories are increasingly adopting automated platform assays.
objectiveWe assessed the performance of commonly used automated immunoassays, with that of LC-MS/MS, and the National Institute of Standards and Technology (NIST) reference values, to measure 25OHD levels. METHODS/
settingWe compared serum 25OHD levels obtained from 219 elderly subjects, enrolled in a vitamin D trial, using the Diasorin Liaison platform assay, and the tandem LC-MS/MS method. We also assessed the performance of the Diasorin and Roche automated assays, expressed as mean % bias from the NIST standards, based on the vitamin D External Quality Assessment Scheme (DEQAS) reports, from 2013 to 2017.
resultsSerum 25OHD levels were significantly lower in the Diasorin compared to LC-MS/MS assay at baseline, 18.5 ± 7.8 vs 20.5 ± 7.6 ng/ml (p < 0.001), and all other time points. Diasorin (25OHD) = 0.76 × LC-MS/MS (25OHD) + 4.3, R
conclusionThe performance of most widely used automated assays is sub-optimal. Our findings underscore the pressing need to re-consider current practices with regard to 25OHD measurements, interpretation of results from research studies, meta-analyses, the development of vitamin D guidelines, and their relevance to optimizing health.
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