ArticleCytotechnology2018
Efficient immortalization of cells derived from critically endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus) with expression of mutant CDK4, Cyclin D1, and telomerase reverse transcriptase.
Article in Cytotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Engineering Immortalized Bovine Granulosa Cells Using a Triple-Gene Approach: Mutant CDK4, Cyclin D1, and TERT.Cell biology international · 2026Article
- Article
- Immortalization of primary cells derived from the endangered Ryukyu long-furred rat.In vitro cellular & developmental biology. Animal · 2023Article
- Immortalized Canine Adipose-Derived Mesenchymal Stem Cells as a Novel Candidate Cell Source for Mesenchymal Stem Cell Therapy.International journal of molecular sciences · 2023Article
- Lentiviral expression of calpain-1 C2-like domain peptide prevents glutamate-induced cell death in mouse hippocampal neuronal HT22 cells.In vitro cellular & developmental biology. Animal · 2022Article
- Immortalization of cells derived from domestic dogs through expressing mutant cyclin-dependent kinase 4, cyclin D1, and telomerase reverse transcriptase.Cytotechnology · 2022Article
- KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media.The journal of poultry science · 2021Article
- Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters.Cytotechnology · 2021Article
- The transcriptome of wild-type and immortalized corneal epithelial cells.Scientific data · 2021Article
- Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor.Frontiers in cell and developmental biology · 2020Article
- Article
- Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon).PloS one · 2019Article
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9 authors.
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Abstract
Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.
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