ArticleMolecular biology reports2018
Expression and characterisation of a thermophilic endo-1,4-β-glucanase from Sulfolobus shibatae of potential industrial application.
Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- Structural and functional insights into extreme thermal stability and activity of two GH12 domains of a multidomain glycosidase from a hyperthermophilic euryarchaeon.The FEBS journal · 2025Article
- Insights into the Lignocellulose-Degrading Enzyme System Based on the Genome Sequence ofInternational journal of molecular sciences · 2025Article
- Hydrolysis of ionic liquid-treated substrate with an Iocasia fonsfrigidae strain SP3-1 endoglucanase.Applied microbiology and biotechnology · 2024Article
- Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing.Engineering microbiology · 2024Review
- Acidophilic heterotrophs: basic aspects and technological applications.Frontiers in microbiology · 2024Review
- Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization.Microorganisms · 2023Review
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- Glycoside Hydrolases and Glycosyltransferases from Hyperthermophilic Archaea: Insights on Their Characteristics and Applications in Biotechnology.Biomolecules · 2021Review
- Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective.Frontiers in bioengineering and biotechnology · 2021Review
- Biomass-degrading glycoside hydrolases of archaeal origin.Biotechnology for biofuels · 2020Review
- Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4.BMC biotechnology · 2019Article
- Novel Hyperthermophilic CrenarchaeonFrontiers in microbiology · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
An endo-1,4-β-D-glucanase gene was cloned from the thermophilic archaea Sulfolobus shibatae and expressed in E. coli. The recombinant enzyme was purified by heat denaturation and affinity chromatography prior to characterisation. The purified recombinant enzyme exhibited maximum activity at 95-100 °C and displayed a broad pH profile with over 91% of its maximum activity observed at pH 3-5. Upon assessment of enzyme thermal stability, full activity was observed after 1 h incubation at 75, 80 and 85 °C while 98%, 90% and 84% of original activity was detected after 2 h at 75, 80 and 85 °C, respectively. Maximum activity was observed on barley β-glucan and lichenan and the purified enzyme also hydrolysed CMC and xylan. Endoglucanase activity was confirmed by viscometric assay with a rapid decrease in substrate viscosity observed immediately upon incubation with barley β-glucan or CMC. The crude enzyme released reducing sugars from acid-pretreated straw at 75-85 °C. The thermophilic nature and biochemical properties of the enzyme indicate its potential suitability in industrial applications undertaken at high temperature, such as the production of second-generation bioethanol from lignocellulosic feedstocks and in the brewing industry. This is the first known report of an endoglucanase from S. shibatae.
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