Evidence map›Paper›PMID 30225356›Full record

ArticleeNeuro

Early Targeting of L-Selectin on Leukocytes Promotes Recovery after Spinal Cord Injury, Implicating Novel Mechanisms of Pathogenesis.

D A McCreedy, S Lee, C J Sontag, P Weinstein, A D Olivas, A F Martinez, T M Fandel, A Trivedi, C A Lowell, S D Rosen and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

D A McCreedyDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
S LeeDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.ORCID 0000-0001-5371-6633
C J SontagDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
P WeinsteinDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
A D OlivasDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
A F MartinezDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
T M FandelDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
A TrivediDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.
C A LowellDepartment of Laboratory Medicine, University of California, San Francisco, CA 94143.ORCID 0000-0002-0467-7073
S D RosenDepartment of Anatomy and Program in Immunology, University of California, San Francisco, CA 94143.ORCID 0000-0002-6245-701X
L J Noble-HaeussleinDepartment of Neurological Surgery, University of California, San Francisco, CA 94143.ORCID 0000-0002-2653-7047
University of California, San Francisco · USGladstone Institutes · US

Funding

ROLE OF MATRIX METALLOPROTEINASES IN SPINAL CORD INJURYR01NS039278 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI NOBLE, LINDA J. · 1999 to 2010
$3.4M
Regulation of Innate Immune Signaling by Lyn KinaseR01AI113272 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOWELL, CLIFFORD A · 2015 to 2019
$2.0M
The role of L-selectin in leukocyte recruitment and longer term recovery after spinal cord injuryF32NS096883 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MCCREEDY, DYLAN A. · 2016 to 2018
$174k
NIAID NIH HHS R01 AI113272NINDS NIH HHS F32 NS096883NINDS NIH HHS R01 NS039278
6 · The paper itself

Abstract

L-selectin, a lectin-like receptor on all leukocyte classes, functions in adhesive and signaling roles in the recruitment of myeloid cells from the blood to sites of inflammation. Here, we consider L-selectin as a determinant of neurological recovery in a murine model of spinal cord injury (SCI). Spinal cord-injured, L-selectin knock-out (KO) mice (male) showed improved long-term recovery with greater white matter sparing relative to wild-type (WT) mice and reduced oxidative stress in the injured cord at 72 h post-SCI. There was a partial and transient reduction in accumulation of neutrophils in the injured spinal cords of KOs at 24 h post-injury. To complement these findings with KO mice, we sought a pharmacologic means for lowering L-selectin levels. We found that diclofenac, a nonsteroidal anti-inflammatory drug (NSAID), induced the shedding of L-selectin from the cell surface of myeloid subsets, specifically neutrophils and non-classical monocytes, in the blood and the injured spinal cord. Diclofenac administration to injured WT mice enhanced neurological recovery to a level comparable to that of KOs but did not improve recovery in KOs. While diclofenac treatment had no effect on myeloid cell accumulation, there was a reduction in oxidative stress at 72 h post-SCI. These findings implicate L-selectin in secondary pathogenesis beyond a role in leukocyte recruitment and raise the possibility of repurposing diclofenac for the treatment of SCI.

Indexed as

InflammationSpinal Cord InjuriesAnimalsAnti-Inflammatory Agents, Non-SteroidalDiclofenacDisease Models, AnimalLeukocytesL-SelectinMaleMiceMice, Inbred C57BLMice, KnockoutMyeloid CellsOxidative StressAnti-Inflammatory Agents, Non-SteroidalDiclofenacL-SelectindiclofenacleukocytesL-selectinmyelinoxidative stressspinal cord injury

Identifiers

PMID30225356
PMCPMC6140118
OpenAlexW2886220552

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.