Evidence map›Paper›PMID 30224665›Full record

ArticleScientific reports2018

MicroRNAs delivery into human cells grown on 3D-printed PLA scaffolds coated with a novel fluorescent PAMAM dendrimer for biomedical applications.

Alessandro Paolini, Luca Leoni, Ilaria Giannicchi, Zeinab Abbaszadeh, Valentina D'Oria, Francesco Mura, Antonella Dalla Cort, Andrea Masotti

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 32 citations in OpenAlex.

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  9. 3D printing applications in bone tissue engineering.Journal of clinical orthopaedics and trauma · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Alessandro PaoliniBambino Gesù Children's Hospital-IRCCS, Research Laboratories, V.le di San Paolo 15, 00146, Rome, Italy. alessandro.paolini@opbg.net.
Luca LeoniDepartment of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
Ilaria GiannicchiDepartment of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
Zeinab AbbaszadehBambino Gesù Children's Hospital-IRCCS, Research Laboratories, V.le di San Paolo 15, 00146, Rome, Italy.
Valentina D'OriaBambino Gesù Children's Hospital-IRCCS, Research Laboratories, V.le di San Paolo 15, 00146, Rome, Italy.
Francesco MuraCenter for the Nanotechnology applied to the Engineering of La Sapienza (CNIS), Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
Antonella Dalla CortDepartment of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
Andrea MasottiBambino Gesù Children's Hospital-IRCCS, Research Laboratories, V.le di San Paolo 15, 00146, Rome, Italy. andrea.masotti@opbg.net.
Sapienza University of Rome · ITIstituti di Ricovero e Cura a Carattere Scientifico · ITBambino Gesù Children's Hospital · IT

Funding

Ministero della Salute (Ministry of Health, Italy) PE-2011-02347026
6 · The paper itself

Abstract

Many advanced synthetic, natural, degradable or non-degradable materials have been employed to create scaffolds for cell culture for biomedical or tissue engineering applications. One of the most versatile material is poly-lactide (PLA), commonly used as 3D printing filament. Manufacturing of multifunctional scaffolds with improved cell growth proliferation and able to deliver oligonucleotides represents an innovative strategy for controlled and localized gene modulation that hold great promise and could increase the number of applications in biomedicine. Here we report for the first time the synthesis of a novel Rhodamine derivative of a poly-amidoamine dendrimer (G = 5) able to transfect cells and to be monitored by confocal microscopy that we also employed to coat a 3D-printed PLA scaffold. The coating do not modify the oligonucleotide binding ability, toxicity or transfection properties of the scaffold that is able to increase cell proliferation and deliver miRNA mimics (i.e., pre-mir-503) into human cells. Although further experiments are required to optimize the dendrimer/miRNA ratio and improve transfection efficiency, we demonstrated the effectiveness of this promising and innovative 3D-printed transfection system to transfer miRNAs into human cells for future biomedical applications.

Indexed as

Printing, Three-DimensionalBiocompatible MaterialsCell ProliferationDendrimersFluorescent DyesHeLa CellsHumansMicroRNAsPolyestersRhodaminesTissue ScaffoldsBiocompatible MaterialsDendrimersFluorescent DyesMicroRNAsPAMAM StarburstPolyesterspoly(lactide)rhodamine BRhodamines

Identifiers

PMID30224665
PMCPMC6141561
OpenAlexW2890155059

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.