Trial reportJournal of immunological methods2018
Effect of delayed cell processing and cryopreservation on immunophenotyping in multicenter population studies.
Trial report in Journal of immunological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 49 citations in OpenAlex.
- Immunomodulatory effects of colchicine on peripheral blood mononuclear cell subpopulations in human obesity: Data from a randomized controlled trial.Obesity (Silver Spring, Md.) · 2023Trial
- Comprehensive flow cytometric reference intervals of leukocyte subsets from six study centers across Europe.Clinical and experimental immunology · 2020Trial
- Associations among discrimination, genetic susceptibility to inflammation, and C-reactive protein.Brain, behavior, & immunity - health · 2026Article
- Peripheral immunosenescence biomarkers and longitudinal cognitive decline: a large population-based study.npj aging · 2026Article
- Immune cell subsets linking insomnia trajectories and incident cognitive impairment: a prospective cohort study.BMC medicine · 2026Article
- Discrimination exposure and lymphocyte differentiation: Results from the health and retirement study.Brain, behavior, & immunity - health · 2026Article
- Adaptive immune cells and kidney function in older adults: sex-specific associations in the health and retirement study (HRS).Frontiers in aging · 2026Article
- Discrimination and dendritic cell abundance among older adults in the health and retirement study.Brain, behavior, and immunity · 2025Article
- "Recommendations for clinical study protocols for immune and inflammatory profiling in Parkinson's disease".NPJ Parkinson's disease · 2025Review
- Evidence for an energetic trade-off model linking inflammaging and immunosenescence in the US Health and Retirement Study and UK Biobank.bioRxiv : the preprint server for biology · 2025Article
- Associations of Immune Cell Subsets With Coronary Artery Calcium Incidence and Progression in the Multi-Ethnic Study of Atherosclerosis.Journal of the American Heart Association · 2025Observational
- Perceived discrimination and monocyte abundance in older adults: Findings from the Health and Retirement Study.Psychoneuroendocrinology · 2025Article
- Rationale and design of the Dog Aging Project precision cohort: a multi-omic resource for longitudinal research in geroscience.GeroScience · 2025Article
- The aging immune system and all-cause mortality in older americans: differences across sex and race/ethnicity.Immunity & ageing : I & A · 2025Article
- The Association of Circulating Immune Cells With Cognitive Function, Brain Imaging, and Incident All-cause and Alzheimer's Dementia: The Framingham Offspring Study.The journals of gerontology. Series A, Biological sciences and medical sciences · 2025Article
- Compositional analysis of lymphocytes and their relationship with health outcomes: findings from the health and retirement study.Immunity & ageing : I & A · 2025Article
- Examining the relationship between biomarkers of immune aging and prevalent physical disability in the health and retirement study.Brain, behavior, and immunity · 2025Article
- Hazards of lunar surface exploration: determining the immunogenicity/allergenicity of lunar dust.Frontiers in immunology · 2025Article
- Associations of circulating T-cell subsets with carotid artery stiffness: the multiethnic study of atherosclerosis.American journal of physiology. Heart and circulatory physiology · 2025Article
- Associations of circulating T-cell subsets in carotid artery stiffness: the Multi-Ethnic Study of Atherosclerosis.medRxiv : the preprint server for health sciences · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Variability induced by delayed cell processing and cell cryopreservation presents unique challenges for immunophenotyping in large population studies. We conducted a pilot study to evaluate the effect of delayed cell processing and cryopreservation on cell percentages obtained by immunophenotyping. We collected blood from 20 volunteers and compared the effect of (a) delayed cell processing up to 72 h (b) cryopreservation and (c) the combined effect of delayed cell processing and cryopreservation on immunophenotyping of 31 cell subsets that included several subsets of T, B, Natural Killer (NK) cells, monocytes and dendritic cells using both whole blood collected in EDTA tubes and peripheral blood mononuclear cells collected in CPT tubes. We found the delayed cell processing up to 72 h or cryopreservation alone did not significantly affect the percentages T cells, dendritic cells or monocytes but significantly increased the percentage of B cells and NK cells (p for trend ≤0.01) but. However combination of delayed cell processing up to 72 h and cryopreservation significantly increased the percentage of T cells as compared to cells processed immediately (p for trend <0.0001) while a delayed cell processing followed by cryopreservation decreased the percentage of NK cells (p for trend <0.0001). Total B-cells increased significantly with a 24-48 h delay in cell processing and cryopreservation but not at 72 h. The percentages of monocytes and dendritic cells remained unaffected by the combination of delayed cell processing and cryopreservation. These findings suggest that immunophenotyping of several immune cell subsets can be successfully implemented in large population studies as long as blood is processed within 48 h of biospecimen collection though some cell subsets may be more susceptible to a combination of delayed cell processing and cryopreservation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.