Evidence map›Paper›PMID 30221931›Full record

ReviewChemical reviews2018

Switchable Fluorophores for Single-Molecule Localization Microscopy.

Honglin Li, Joshua C Vaughan

Abstract readHistorical ArticleReview
In one paragraph

Review in Chemical reviews, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.

0numbers the graph read from it
0cells of the map it votes in
106citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

106 citing papers in PubMed.

  1. Article
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  17. On-Target Photoassembly of Pyronin Dyes for Super-Resolution Microscopy.Angewandte Chemie (International ed. in English) · 2025
    Article
  18. Review
  19. Article
  20. Photoactivation of BODIPY Fluorescence with Green Light.The Journal of organic chemistry · 2025
    Article

46 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Honglin Li

Funding

Spatially Resolved Transcriptomics Enabled by Ultrabright Pdot Probes for Interrogation of Complex TissuesR01MH115767 · NIMH · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T, VAUGHAN, JOSHUA · 2017 to 2021
$3.7M
NIMH NIH HHS R01 MH115767Wellcome Trust
6 · The paper itself

Abstract

The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.

Indexed as

AnimalsCell Line, TumorFluorescent DyesHistory, 20th CenturyHistory, 21st CenturyHumansLuminescent ProteinsMicroscopy, FluorescenceOrganic ChemicalsQuantum DotsSingle Molecule ImagingFluorescent DyesLuminescent ProteinsOrganic Chemicals

Identifiers

PMID30221931
PMCPMC6476321

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.