ArticleMolecular medicine reports2018
Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro.
Article in Molecular medicine reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies.Clinical oral investigations · 2020Pooled it
- A scoping review on platelet-rich fibrin-driven bone regeneration: biological mechanisms and clinical applications in oral and maxillofacial surgery.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2026Article
- Effects of advanced platelet-rich fibrin combined with a collagen membrane on human periodontal ligament stem cells at two extract concentrations: an in vitro study.BMC oral health · 2026Article
- Effects of Nano-Hydroxyapatite-Coated PRF on Gingiva-Derived Mesenchymal Stem Cells: In Vitro Study.International journal of molecular sciences · 2026Article
- Effect of tube cooling on the osteogenic activity of concentrated platelet-rich fibrin in human periodontal ligament cells.Biomaterial investigations in dentistry · 2026Article
- Evaluation of antimicrobial activity of sutures soaked in injectable platelet rich fibrin or platelet rich fibrin lysate against common oral pathogens: A pilot study.Journal of advanced periodontology & implant dentistry · 2025Article
- Assessing healing of periapical pathologies by platelet-rich fibrin: A clinical study.Bioinformation · 2025Article
- Article
- Platelet-Rich Fibrin Increases CXCL8 Expression in Gingival Fibroblasts.Biomedicines · 2024Article
- Plasma Rich in Growth Factors in Bone Regeneration: The Proximity to the Clot as a Differential Factor in Osteoblast Cell Behaviour.Dentistry journal · 2024Article
- L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro.Biomedicines · 2024Article
- Osteogenic potential of apical papilla stem cells mediated by platelet-rich fibrin and low-level laser.Odontology · 2024Article
- Article
- The Effects of Platelet-Rich Fibrin in the Behavior of Mineralizing Cells Related to Bone Tissue Regeneration-A Scoping Review of In Vitro Evidence.Journal of functional biomaterials · 2023Article
- Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study.Journal of dentistry (Shiraz, Iran) · 2023Article
- Low-intensity pulsed ultrasound enhances immunomodulation and facilitates osteogenesis of human periodontal ligament stem cells by inhibiting the NF-κB pathway.Cell and tissue banking · 2023Article
- Clinical application of platelet-rich fibrin to enhance dental implant stability: A systematic review and meta-analysis.Heliyon · 2023Article
- Effects of advanced platelet-rich fibrin combined with xenogenic bone on human periodontal ligament stem cells.Clinical and experimental dental research · 2022Article
- Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro.Scientific reports · 2021Article
- [Effect of concentrated growth factor combined with mineralized collagen material on the adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells and the osteogenic effectZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of the present study was to evaluate the effects of platelet‑rich fibrin (PRF) exudate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells (hPDLCs) in vitro. In the present study PRF was obtained with permission, from the peripheral blood of healthy donors and PRF exudates were collected on the 7th day of incubation. hPDLCs were obtained from healthy premolars, cultured by a tissue explant method and identified with anti‑vimentin and anti‑cytokeratin antibodies. PRF exudates were added to hPDLCs in different concentrations to evaluate cell proliferation and osteogenic differentiation. The proliferation of hPDLCs was measured using a colorimetric assay. Osteogenic differentiation and mineralization were determined by Alizarin red staining, alkaline phosphatase activity (ALP), western blotting and reverse transcription‑quantitative polymerase chain reaction. Cell proliferation was enhanced by addition of the PRF exudate, which also promoted the formation of mineralized matrix nodules and upregulated ALP activity and osteoblast‑associated levels of osteocalcin, runt‑related transcription factor and osterix gene expression. As these stimulatory effects occurred in a dose‑dependent manner, it was concluded that high concentrations of the PRF exudate served an essential role in the proliferation, osteogenic differentiation and mineralization of hPDLCs in vitro. The present study demonstrated that PRF exudate enhanced hPDLC proliferation, induced the osteoblastic differentiation of hPDLCs into mineralized tissue‑formation cells in vitro, and may therefore provide potential benefits for periodontal tissue engineering; contributing to the primary processes of periodontal tissue regeneration. From the perspective of both economics and biology, PRF has greater clinical benefits than analogous growth factors.
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