ArticleJournal of clinical laboratory analysis2019
Biotin interference in TSH, FT4, and FT3 assays based on the LOCI technology: Identifying interference by dilution.
Article in Journal of clinical laboratory analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 6 citations in OpenAlex.
- Comparison of Enzyme-Linked Immunosorbent Assay and Lateral Flow Assay to Measure Thyroid-Stimulating Hormone and Free T4 in Human Serum.Journal of clinical laboratory analysis · 2025Article
- Biotin interference in TSH, FT4, and FT3 assays based on the LOCI technology: Identifying interference by dilution.Journal of clinical laboratory analysis · 2019Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
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Abstract
backgroundAlthough biotin interferences in TSH, FT3, FT4, and other biotinylated antibody-based assays manufactured by Roche Diagnostics have been well studied, there are relatively few reports on biotin interference in biotin-based assays manufactured by other companies. We investigated biotin interferences in TSH, FT4, and FT3 assays based on the LOCI (luminescent oxygen channeling assay) technology using the Dimension Vista 1500 analyzer (Siemens).
methodsWe prepared four serum pools using leftover specimens. Three serum pools were prepared initially for the original study but the 4
resultsWe observed significantly elevated FT3 levels at biotin concentration of 100 ng/mL. In contrast, FT4 levels were falsely elevated but TSH levels were falsely decreased at a biotin level of 500 ng/mL. We also observed nonlinearity in dilution experiment.
conclusionsWe conclude that FT3 assay is most susceptible to biotin interference (threshold: 100 ng/mL) while the FT4 and TSH assays are less affected (threshold: 500 ng/mL). In addition, we also observed nonlinearity upon 1:3 dilution, which may indicate biotin interference (or interference from other compounds).
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