ArticleNucleic acids research2018
A synthetic free fatty acid-regulated transgene switch in mammalian cells and mice.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- A Clozapine-Responsive GPCR-Based Gene Switch for Pharmacological Control of Gene Expression in Mammalian Cells and In Vivo.International journal of molecular sciences · 2026Article
- Integrated transcriptomics, proteomics, and metabolomics analysis reveals newcastle disease virus reshapes glycerophospholipid metabolism.BMC genomics · 2026Article
- Regulation of therapeutic protein release in response to circadian biomarkers.Nature communications · 2025Article
- Preparation, Characterization, and Hepatoprotective Activity Evaluation of Quercetin-loaded PluronicCurrent drug delivery · 2025Article
- Synthetic Gene Circuits for Regulation of Next-Generation Cell-Based Therapeutics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Engineering natural molecule-triggered genetic control systems for tunable gene- and cell-based therapies.Synthetic and systems biotechnology · 2023Article
- Article
- Biomarker-driven feedback control of synthetic biology systems for next-generation personalized medicine.Frontiers in bioengineering and biotechnology · 2022Review
- Synthetic biology as driver for the biologization of materials sciences.Materials today. Bio · 2021Review
- Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05Bioprocess and biosystems engineering · 2021Article
- Precision Tools in Immuno-Oncology: Synthetic Gene Circuits for Cancer Immunotherapy.Vaccines · 2020Review
- Building sophisticated sensors of extracellular cues that enable mammalian cells to work as "doctors" in the body.Cellular and molecular life sciences : CMLS · 2020Review
- Engineering and Rewiring of a Calcium-Dependent Signaling Pathway.ACS synthetic biology · 2020Article
- Rewiring of endogenous signaling pathways to genomic targets for therapeutic cell reprogramming.Nature communications · 2020Article
- Myristic Acid Coated Protein Immobilised Mesoporous Silica Particles as pH Induced Oral Delivery System for the Delivery of Biomolecules.Pharmaceuticals (Basel, Switzerland) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
Abstract
Trigger-inducible transgene expression systems are utilized in biopharmaceutical manufacturing and also to enable controlled release of therapeutic agents in vivo. We considered that free fatty acids (FFAs), which are dietary components, signaling molecules and important biomarkers, would be attractive candidates as triggers for novel transgene switches with many potential applications, e.g. in future gene- and cell-based therapies. To develop such a switch, we rewired the signal pathway of human G-protein coupled receptor 40 to a chimeric promoter triggering gene expression through an increase of intracellular calcium concentration. This synthetic gene switch is responsive to physiologically relevant FFA concentrations in different mammalian cell types grown in culture or in a bioreactor, or implanted into mice. Animal recipients of microencapsulated sensor cells containing this switch exhibited significant transgene induction following consumption of dietary fat (such as Swiss cheese) or under hyperlipidaemic conditions, including obesity, diabetes and lipodystrophy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.