Evidence map›Paper›PMID 30218404›Full record

ArticleCellular and molecular neurobiology2018

A Novel Microfluidic Device-Based Neurite Outgrowth Inhibition Assay Reveals the Neurite Outgrowth-Promoting Activity of Tropomyosin Tpm3.1 in Hippocampal Neurons.

Holly Stefen, Amin Hassanzadeh-Barforoushi, Merryn Brettle, Sandra Fok, Alexandra K Suchowerska, Nicodemus Tedla, Tracie Barber, Majid Ebrahimi Warkiani, Thomas Fath

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PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular neurobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Holly StefenNeurodegenerative and Repair Unit, School of Medical Science, UNSW Sydney, Sydney, NSW, Australia.
Amin Hassanzadeh-BarforoushiSchool of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
Merryn BrettleNeurodegenerative and Repair Unit, School of Medical Science, UNSW Sydney, Sydney, NSW, Australia.
Sandra FokNeurodegenerative and Repair Unit, School of Medical Science, UNSW Sydney, Sydney, NSW, Australia.
Alexandra K SuchowerskaNeurodegenerative and Repair Unit, School of Medical Science, UNSW Sydney, Sydney, NSW, Australia.
Nicodemus TedlaInflammation Research, School of Medical Sciences, University of New South Wales, Sydney, NSW, Australia.
Tracie BarberSchool of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
Majid Ebrahimi WarkianiSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia. majid.warkiani@uts.edu.au.
Thomas FathNeurodegenerative and Repair Unit, School of Medical Science, UNSW Sydney, Sydney, NSW, Australia. thomas.fath@mq.edu.au.ORCID http://orcid.org/0000-0002-6877-7567
UNSW Sydney · AUGarvan Institute of Medical Research · AUUniversity of Technology Sydney · AU

Funding

Australian Research Council (AU) DP170103704Australian Research Council (AU) DP180101473National Health and Medical Research Council (AU) APP1083209
6 · The paper itself

Abstract

Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.

Indexed as

Lab-On-A-Chip DevicesNeuronal OutgrowthAnimalsHippocampusHumansNeuronsNogo ProteinsReproducibility of ResultsTropomyosinNogo ProteinsTropomyosinMicrofluidic systemsNeurite outgrowth inhibitionNogoATropomyosins

Identifiers

PMID30218404
PMCPMC11469800
OpenAlexW2889625403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.