Evidence map›Paper›PMID 30218351›Full record

ArticleMolecular biology reports2018

New polymorphic microsatellite loci revealed for the dusky shark Carcharhinus obscurus through Ion Proton double-digest RAD sequencing.

Simo N Maduna, Charné Rossouw, Ruhan Slabbert, Sabine P Wintner, Charlene da Silva, Aletta E Bester-van der Merwe

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Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Simo N MadunaMolecular Breeding and Biodiversity Group, Department of Genetics, Stellenbosch University, Private Bag XI, Stellenbosch, 7602, South Africa. simo@sun.ac.za.ORCID http://orcid.org/0000-0002-9372-4360
Charné RossouwMolecular Breeding and Biodiversity Group, Department of Genetics, Stellenbosch University, Private Bag XI, Stellenbosch, 7602, South Africa.ORCID http://orcid.org/0000-0002-7676-6809
Ruhan SlabbertRoche Sequencing Solutions Cape Town, 271 Victoria Road, Salt River, Cape Town, 7925, South Africa.ORCID http://orcid.org/0000-0002-7368-3274
Sabine P WintnerKwaZulu-Natal Sharks Board, Private Bag 2, Umhlanga Rocks, 4320, South Africa.
Charlene da SilvaFisheries Research, Department of Agriculture, Forestry and Fisheries, Private Bag X2, Rogge Bay, 8012, South Africa.
Aletta E Bester-van der MerweMolecular Breeding and Biodiversity Group, Department of Genetics, Stellenbosch University, Private Bag XI, Stellenbosch, 7602, South Africa.ORCID http://orcid.org/0000-0002-0332-7864

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The non-model shark species, dusky shark Carcharhinus obscurus, is a bio-economically and recreationally important shark in many areas of its range. Despite of the fishery importance of C. obscurus few genetic resources are currently available for the species. Here, we report on the isolation of eight novel microsatellite loci from C. obscurus using a double-digest restriction site associated DNA (RAD) sequencing approach on the Ion Proton semiconductor platform (ddRADseq-ion). We characterised the loci in 26 individuals and all loci were polymorphic, exhibiting 5-10 alleles (average 6.6), and observed and expected heterozygosities of 0.385-0.962 and 0.479-0.847, respectively. We found that all pairs of loci were in linkage equilibrium and conformed to Hardy-Weinberg expectations. The loci reported in this study are only the second set of microsatellite loci ever characterized for C. obscurus and will be valuable for molecular ecology studies for this vulnerable species.

Indexed as

AllelesAnimalsGenetic LociHeterozygoteLinkage DisequilibriumMicrosatellite RepeatsPolymorphism, GeneticRestriction MappingSequence Analysis, DNASharksCarcharhinus obscurusDouble-digestDusky sharkIon ProtonIon TorrentMicrosatellitesPopulation geneticsRAD sequencing

Identifiers

PMID30218351

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.