ArticleWorld journal of microbiology & biotechnology2018
Agrobacterium-mediated transformation of the ascomycete mushroom Morchella importuna using polyubiquitin and glyceraldehyde-3-phosphate dehydrogenase promoter-based binary vectors.
Article in World journal of microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 14 citations in OpenAlex.
- Engineering a high-efficiency expression system in Tremella fuciformis using novel promoters and optimized transformation conditions.World journal of microbiology & biotechnology · 2026Article
- The Transformation and Protein Expression of the Edible MushroomJournal of fungi (Basel, Switzerland) · 2025Article
- Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects.Journal of fungi (Basel, Switzerland) · 2024Review
- Large-Scale Field Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Review
- Mating-Type Genes Play an Important Role in Fruiting Body Development inJournal of fungi (Basel, Switzerland) · 2022Article
- Construction of nucleus-directed fluorescent reporter systems and its application to verification of heterokaryon formation inFrontiers in microbiology · 2022Article
- A comprehensive review on Morchella importuna: cultivation aspects, phytochemistry, and other significant applications.Folia microbiologica · 2021Review
- Functional Analysis of a NovelJournal of fungi (Basel, Switzerland) · 2020Article
- The development of an efficient RNAi system based on Agrobacterium-mediated transformation approach for studying functional genomics in medical fungus Wolfiporia cocos.World journal of microbiology & biotechnology · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Morchella importuna is a worldwide distributed edible mushroom with high ecological and economic values, but the molecular and genetic research about this mushroom has been hindered due to lack of an efficient transformation method. Here, we report for the first time the successful transformation of M. importuna by using a hypervirulent Agrobacterium tumefaciens strain bearing the constructed binary plasmid p1391-U-GUS. The selectable markers used were the genes for hygromycin resistance under the control of the polyubquitin promoter from M. importuna. The reporter genes were those for enhanced green fluorescent protein (EGFP) and the β-Glucuronidase (GUS) under the control of glyceraldehyde-3-phosphate dehydrogenase promoter and polyubquitin promoter respectively. The presence of the reporter gene EGFP in the transformants was confirmed by the fluorescence and confocal microscope and molecular analysis and that of the reporter gene GUS was verified by enzyme activity and molecular analysis. The analysis results of both reporter genes indicated that Agrobacterium-mediated transformation was successfully performed in M. importuna.
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