ArticleNature protocols2018
Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Advances in nanomechanical property mapping by atomic force microscopy.Nanoscale advances · 2025Review
- Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker.ACS nano · 2025Article
- Incongruous Harmonics of Vibrating Solid-Solid Interface.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Advances in microfluidic platforms for tumor cell phenotyping: from bench to bedside.Lab on a chip · 2025Review
- The mechanopathology of the tumor microenvironment: detection techniques, molecular mechanisms and therapeutic opportunities.Frontiers in cell and developmental biology · 2025Review
- Machine learning and artificial intelligence: Enabling the clinical translation of atomic force microscopy-based biomarkers for cancer diagnosis.Computational and structural biotechnology journal · 2024Review
- Multi-Channel Signals in Dynamic Force-Clamp Mode of Microcantilever Sensors for Detecting Cellular Peripheral Brush.Sensors (Basel, Switzerland) · 2024Article
- Beyond stiffness: Multiscale viscoelastic features as biomechanical markers for assessing cell types and states.Biophysical journal · 2024Article
- Mechanical properties of human tumour tissues and their implications for cancer development.Nature reviews. Physics · 2024Article
- Cell Surface Parameters for Accessing Neutrophil Activation Level with Atomic Force Microscopy.Cells · 2024Article
- Eukaryotic CRFK Cells Motion Characterized with Atomic Force Microscopy.International journal of molecular sciences · 2022Article
- 3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers.Scientific reports · 2022Article
- Viscoelastic parameterization of human skin cells characterize material behavior at multiple timescales.Communications biology · 2022Article
- Review
- Reciprocal regulation of cellular mechanics and metabolism.Nature metabolism · 2021Review
- Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes.Nature protocols · 2021Article
- Data acquisition and imaging using wavelet transform: a new path for high speed transient force microscopy.Nanoscale advances · 2021Article
- Viscoelasticity and Volume of Cortical Neurons under Glutamate Excitotoxicity and Osmotic Challenges.Biophysical journal · 2020Article
- Viscoelasticity in simple indentation-cycle experiments: a computational study.Scientific reports · 2020Article
- Wideband Magnetic Excitation System for Atomic Force Microscopy Cantilevers with Megahertz-Order Resonance Frequency.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The goal of mechanobiology is to understand the links between changes in the physical properties of living cells and normal physiology and disease. This requires mechanical measurements that have appropriate spatial and temporal resolution within a single cell. Conventional atomic force microscopy (AFM) methods that acquire force curves pointwise are used to map the heterogeneous mechanical properties of cells. However, the resulting map acquisition time is much longer than that required to study many dynamic cellular processes. Dynamic AFM (dAFM) methods using resonant microcantilevers are compatible with higher-speed, high-resolution scanning; however, they do not directly acquire force curves and they require the conversion of a limited number of instrument observables to local mechanical property maps. We have recently developed a technique that allows commercial AFM systems equipped with direct cantilever excitation to quantitatively map the viscoelastic properties of live cells. The properties can be obtained at several widely spaced frequencies with nanometer-range spatial resolution and with fast image acquisition times (tens of seconds). Here, we describe detailed procedures for quantitative mapping, including sample preparation, AFM calibration, and data analysis. The protocol can be applied to different biological samples, including cells and viruses. The transition from dAFM imaging to quantitative mapping should be easily achievable for experienced AFM users, who will be able to set up the protocol in <30 min.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.