ArticleBlood advances2018
Blood manufacturing methods affect red blood cell product characteristics and immunomodulatory activity.
Article in Blood advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Additive effects of blood donor smoking and gamma irradiation on outcome measures of red blood cell transfusion.Transfusion · 2020Trial
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- Short- and long-term effects of transfusion in β-thalassemia: a longitudinal study of transfusion efficiency factors.Blood advances · 2026Article
- Hemoglobin-oxygen affinity changes in neonatal blood transfusions: RBC selection insights.Pediatric research · 2025Article
- Transfused Red Blood Cell Characteristics and Kidney Transplant Outcomes Among Patients Receiving Early Posttransplant Transfusion.JAMA network open · 2023Article
- Association between red blood cell distribution width and left ventricular hypertrophy in pediatric essential hypertension.Frontiers in pediatrics · 2023Article
- The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward.European journal of translational myology · 2022Article
- Red blood cell distribution width and tumor necrosis factor-α for the early prediction of coronary artery lesion in Kawasaki disease: a retrospective study.European journal of pediatrics · 2022Article
- Exosomes From Packed Red Cells Induce Human Mast Cell Activation and the Production of Multiple Inflammatory Mediators.Frontiers in immunology · 2021Article
- Donor-dependent aging of young and old red blood cell subpopulations: Metabolic and functional heterogeneity.Transfusion · 2020Article
- Red-blood-cell manufacturing methods and storage solutions differentially induce pulmonary cell activation.Vox sanguinis · 2020Article
- Article
- Methodological considerations for linked blood donor-component-recipient analyses in transfusion medicine research.ISBT science series · 2020Article
- Circulating Extracellular Vesicles and Endothelial Damage in Sickle Cell Disease.Frontiers in physiology · 2020Review
- Article
- Post-Transfusion Purpura: Current Perspectives.Journal of blood medicine · 2019Review
- Packed red blood cells inhibit T-cell activation via ROS-dependent signaling pathways.The Journal of biological chemistryArticle
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
Abstract
Transfusion of red cell concentrates (RCCs) is associated with increased risk of adverse outcomes that may be affected by different blood manufacturing methods and the presence of extracellular vesicles (EVs). We investigated the effect of different manufacturing methods on hemolysis, residual cells, cell-derived EVs, and immunomodulatory effects on monocyte activity. Thirty-two RCC units produced using whole blood filtration (WBF), red cell filtration (RCF), apheresis-derived (AD), and whole blood-derived (WBD) methods were examined (n = 8 per method). Residual platelet and white blood cells (WBCs) and the concentration, cell of origin, and characterization of EVs in RCC supernatants were assessed in fresh and stored supernatants. Immunomodulatory activity of RCC supernatants was assessed by quantifying monocyte cytokine production capacity in an in vitro transfusion model. RCF units yielded the lowest number of platelet and WBC-derived EVs, whereas the highest number of platelet EVs was in AD (day 5) and in WBD (day 42). The number of small EVs (<200 nm) was greater than large EVs (≥200 nm) in all tested supernatants, and the highest level of small EVs were in AD units. Immunomodulatory activity was mixed, with evidence of both inflammatory and immunosuppressive effects. Monocytes produced more inflammatory interleukin-8 after exposure to fresh WBF or expired WBD supernatants. Exposure to supernatants from AD and WBD RCC suppressed monocyte lipopolysaccharide-induced cytokine production. Manufacturing methods significantly affect RCC unit EV characteristics and are associated with an immunomodulatory effect of RCC supernatants, which may affect the quality and safety of RCCs.
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