ArticleMicrobial cell factories2018
Metabolic engineering of Corynebacterium glutamicum for anthocyanin production.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 98 citations in OpenAlex.
- Engineering of Corynebacterium glutamicum for the synthesis of aromatic compounds.Applied microbiology and biotechnology · 2025Review
- Assessment of safety:Toxicology reports · 2024Article
- Review of the Proteomics and Metabolic Properties ofMicroorganisms · 2024Review
- De novo biosynthesis of β-arbutin in Corynebacterium glutamicum via pathway engineering and process optimization.Biotechnology for biofuels and bioproducts · 2024Article
- Biosynthesis of Apigenin Glucosides in EngineeredJournal of microbiology and biotechnology · 2024Article
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Review
- Production of bilirubin via whole-cell transformation utilizing recombinant Corynebacterium glutamicum expressing a β-glucuronidase from Staphylococcus sp. RLH1.Biotechnology letters · 2024Article
- A major facilitator superfamily transporter MdtH inApplied and environmental microbiology · 2024Article
- Identification ofFrontiers in microbiology · 2024Article
- Advances in the Preparation, Stability, Metabolism, and Physiological Roles of Anthocyanins: A Review.Foods (Basel, Switzerland) · 2023Review
- Glucosylation of Isoeugenol and Monoterpenes inMolecules (Basel, Switzerland) · 2023Article
- Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production.Metabolites · 2023Review
- Synthetic biology tools for engineeringComputational and structural biotechnology journal · 2023Review
- Construction and Analysis of an Enzyme-Constrained Metabolic Model ofBiomolecules · 2022Article
- Genomic landscapes of bacterial transposons and their applications in strain improvement.Applied microbiology and biotechnology · 2022Review
- Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum.Microbial cell factories · 2022Article
- Oxidative Transformation of Dihydroflavonols and Flavan-3-ols by Anthocyanidin Synthase fromMolecules (Basel, Switzerland) · 2022Article
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Efficient production of anthocyanins inFrontiers in bioengineering and biotechnology · 2022Article
- Colorful Treasure From Agro-Industrial Wastes: A Sustainable Chassis for Microbial Pigment Production.Frontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundAnthocyanins such as cyanidin 3-O-glucoside (C3G) have wide applications in industry as food colorants. Their current production heavily relies on extraction from plant tissues. Development of a sustainable method to produce anthocyanins is of considerable interest for industrial use. Previously, E. coli-based microbial production of anthocyanins has been investigated extensively. However, safety concerns on E. coli call for the adoption of a safe production host. In the present study, a GRAS bacterium, Corynebacterium glutamicum, was introduced as the host strain to synthesize C3G. We adopted stepwise metabolic engineering strategies to improve the production titer of C3G.
resultsAnthocyanidin synthase (ANS) from Petunia hybrida and 3-O-glucosyltransferase (3GT) from Arabidopsis thaliana were coexpressed in C. glutamicum ATCC 13032 to drive the conversion from catechin to C3G. Optimized expression of ANS and 3GT improved the C3G titer by 1- to 15-fold. Further process optimization and improvement of UDP-glucose availability led to ~ 40 mg/L C3G production, representing a > 100-fold titer increase compared to production in the un-engineered, un-optimized starting strain.
conclusionsFor the first time, we successfully achieved the production of the specialty anthocyanin C3G from the comparatively inexpensive flavonoid precursor catechin in C. glutamicum. This study opens up more possibility of C. glutamicum as a host microbe for the biosynthesis of useful and value-added natural compounds.
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Registered trials
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