Evidence map›Paper›PMID 30217078›Full record

ArticleViruses2018

Degenerate PCR Primers to Reveal the Diversity of Giant Viruses in Coastal Waters.

Yanze Li, Pascal Hingamp, Hiroyasu Watai, Hisashi Endo, Takashi Yoshida, Hiroyuki Ogata

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Tara Oceans: towards global ocean ecosystems biology.Nature reviews. Microbiology · 2020
    Review
  14. Article
  15. Article
  16. Targeted metagenomic recovery of four divergent viruses reveals shared and distinctive characteristics of giant viruses of marine eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019
    Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Yanze LiBioinformatics Center, Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan. yanzeli@kuicr.kyoto-u.ac.jp.
Pascal HingampAix Marseille Université, Université de Toulon, CNRS, IRD, MIO UM 110, 13288 Marseille, France. pascal.hingamp@mio.osupytheas.fr.
Hiroyasu WataiGraduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan. hrhsys@gmail.com.
Hisashi EndoBioinformatics Center, Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan. endo@kuicr.kyoto-u.ac.jp.ORCID 0000-0003-0016-1624
Takashi YoshidaGraduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan. yoshiten@kais.kyoto-u.ac.jp.
Hiroyuki OgataBioinformatics Center, Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan. ogata@kuicr.kyoto-u.ac.jp.ORCID 0000-0001-6594-377X
Kyoto University · JPCentre National de la Recherche Scientifique · FR

Funding

Canon Foundation for Scientific Research 203143100025French Investments for the Future program ANR-11-BTBR-0008Institute for Chemical Research, Kyoto University 2016-28Institute for Chemical Research, Kyoto University 2017-25Japan Society for the Promotion of Science 16KT0020Japan Society for the Promotion of Science 17H03850Japan Society for the Promotion of Science 26430184
6 · The paper itself

Abstract

"Megaviridae" is a proposed family of giant viruses infecting unicellular eukaryotes. These viruses are ubiquitous in the sea and have impact on marine microbial community structure and dynamics through their lytic infection cycle. However, their diversity and biogeography have been poorly characterized due to the scarce detection of Megaviridae sequences in metagenomes, as well as the limitation of reference sequences used to design specific primers for this viral group. Here, we propose a set of 82 degenerated primers (referred to as MEGAPRIMER), targeting DNA polymerase genes (

Indexed as

BiodiversityPolymerase Chain ReactionWater MicrobiologyComputational BiologyGenome, ViralGiant VirusesMetagenomeMetagenomicsPhylogenySeawaterB family DNA polymerase (PolB)Megaviridaemeta-barcodemetagenomeMimiviridae

Identifiers

PMID30217078
PMCPMC6163766
OpenAlexW2889885446

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.