Evidence map›Paper›PMID 30217018›Full record

ArticleViruses2018

Identification of Novel Subcellular Localization and Trafficking of HIV-1 Nef Variants from Reference Strains G (F1.93.HH8793) and H (BE.93.VI997).

Logan R Van Nynatten, Aaron L Johnson, Brennan S Dirk, Emily N Pawlak, Rajesh Abraham Jacob, S M Mansour Haeryfar, Jimmy D Dikeakos

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Logan R Van NynattenDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. lvannyna@uwo.ca.
Aaron L JohnsonDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. ajohn228@uwo.ca.
Brennan S DirkDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. bdirk@uwo.ca.
Emily N PawlakDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. epawlak@uwo.ca.
Rajesh Abraham JacobDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. rjacob24@uwo.ca.
S M Mansour HaeryfarDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. mansour.haeryfar@schulich.uwo.ca.
Jimmy D DikeakosDepartment of Microbiology and Immunology, The University of Western Ontario, Schulich School of Medicine and Dentistry, London, ON N6A 5C1, Canada. jimmy.dikeakos@uwo.ca.
Western University · CA

Funding

CIHR 286719CIHR MOP 286719
6 · The paper itself

Abstract

The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef, plays an essential role in disease progression and pathogenesis via hijacking the host cellular membrane-trafficking machinery. Interestingly, HIV-1 group-M subtypes display differences in the rate of disease progression. However, few reports investigated how the cellular behaviors and activities of Nef isolates from reference strains may differ between HIV-1 group-M subtypes. Here, we characterize how differing cellular distributions of Nef proteins across group-M subtypes may impact protein function using immunofluorescence microscopy and flow cytometric analysis. We demonstrate that Nef variants isolated from HIV-1 group-M subtypes display differences in expression, with low expressing Nef proteins from reference strains of subtypes G (F1.93.HH8793) and H (BE.93.VI997) also displaying decreased functionality. Additionally, we demonstrate variations in the subcellular distribution and localization of these Nef proteins. Nef from subtype G (F1.93.HH8793) and H (BE.93.VI997) reference strains also failed to colocalize with the trans-Golgi network, and were not differentially localized to cellular markers of multivesicular bodies or lysosomes. Strikingly, our results demonstrate that HIV-1 Nef proteins from reference strains G (F1.93.HH8793) and H (BE.93.VI997) highly colocalize with labeled mitochondrial compartments.

Indexed as

Genetic VariationAmino Acid SequenceCell LineEndoplasmic ReticulumGene Expression Regulation, ViralGenotypeHeLa CellsHIV-1HIV InfectionsHumansIntracellular SpaceMitochondrianef Gene Products, Human Immunodeficiency VirusProtein Transportnef Gene Products, Human Immunodeficiency Virusnef protein, Human immunodeficiency virus 1diversityhuman immunodeficiency virusmicroscopyNefsubtypes

Identifiers

PMID30217018
PMCPMC6164931
OpenAlexW2890576383

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.