Evidence map›Paper›PMID 30217016›Full record

ArticleInternational journal of molecular sciences2018

Concatenation of Human Connexin26 (hCx26) and Human Connexin46 (hCx46) for the Analysis of Heteromeric Gap Junction Hemichannels and Heterotypic Gap Junction Channels.

Patrik Schadzek, Doris Hermes, Yannick Stahl, Nadine Dilger, Anaclet Ngezahayo

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Patrik SchadzekInstitut für Biophysik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. p.schadzek@biophysik.uni-hannover.de.ORCID 0000-0002-0131-5093
Doris HermesInstitut für Biophysik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. hermes@em.mpg.de.ORCID 0000-0002-1779-093X
Yannick StahlInstitut für Biophysik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. yannick.stahl1@googlemail.com.ORCID 0000-0003-0861-4907
Nadine DilgerInstitut für Biophysik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. n.dilger@biophysik.uni-hannover.de.ORCID 0000-0003-2978-1763
Anaclet NgezahayoInstitut für Biophysik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. ngezahayo@biophysik.uni-hannover.de.
Leibniz University Hannover · DE

Funding

Deutsche Forschungsgemeinschaft Transregio TR37, NG4/10-1
6 · The paper itself

Abstract

Gap junction channels and hemichannels formed by concatenated connexins were analyzed. Monomeric (hCx26, hCx46), homodimeric (hCx46-hCx46, hCx26-hCx26), and heterodimeric (hCx26-hCx46, hCx46-hCx26) constructs, coupled to GFP, were expressed in HeLa cells. Confocal microscopy showed that the tandems formed gap junction plaques with a reduced plaque area compared to monomeric hCx26 or hCx46. Dye transfer experiments showed that concatenation allows metabolic transfer. Expressed in

Indexed as

AnimalsConnexin 26ConnexinsGap JunctionsHeLa CellsHumansIon ChannelsPatch-Clamp TechniquesXenopus laevisConnexin 26ConnexinsGJA3 protein, humanIon Channelschannel stoichiometryconcatenated connexinsdual whole-cell patch-clampdye transfergap junctionheteromeric connexonshuman connexin26human connexin46inside-out patch-clamp configurationoligomerization

Identifiers

PMID30217016
PMCPMC6163895
OpenAlexW2891858989

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.